PowerLab Systems Measure QT Prolongation in Zebrafish
Phylonix, a contract research organization headquartered in Cambridge, MA, develops and markets in vivo zebrafish assays for therapeutic drug screening for research and pre-clinical studies. The zebrafish is extremely cost-effective compared to larger animal models, and its embryo is already established as a powerful model for studying genetics and developmental biology.
Recently, articles validating adult zebrafish as a model for researching drug-induced QT prolongation have appeared in Circulation and AJP Heart and Circulatory Physiology. Zebrafish and human heart electrophysiological properties are comparable, and ECG patterns, including the PR interval, QRS complex and QT interval, are similar.
Phylonix researchers add drugs to the water in the zebrafish’s recording chamber. The ECG signal is acquired using needle electrodes connected to an ADInstruments’ Octal Bio Amp, and PowerLab 8/30 data acquisition unit. The data is recorded and displayed using Chart software. Phylonix electrophysiologist Dr Demian Park has worked closely with ADInstruments staff to optimize their recordings.
The ECG Analysis Module has accelerated Phylonix’s data analysis processes. It automatically detects, analyzes and extracts signal parameters,including the QT interval, in real time. As Phylonix’s recordings are extremely long, Dr Park considers this software “really necessary” for research productivity.
For more information on this or other ADInstruments equipment call 1-888-965-6040.
Education Corner
New Animal Experiment for Chart
Dr. Giovanni Casotti from the West Chester University Department of Biology has created a new experiment entitled, "Animal Metabolism." The experiment was one of the highlights of our education workshop hosted by Dr. Casotti. The new experiment features the use of the PowerLab 4/25 and a CWE gas analyzer and flow meter. Students are introduced to a gas analysis technique for recording respiratory variables and determining metabolic rate. This experiment is now available for download here.
For more information about this or any other ADInstruments education materials please contact Wes Colgan, Education Project Manager at 1-888-965-6040 ext. 201.
Events and Conferences
Events
Nebraska Physiology Society Annual Meeting
September 8th
Nebraska City, NE
AALAS Annual Vendor Night at Columbus Children's Research Institute
September 13th
Columbus, OH
BMES 2007
September 26th - 29th
Los Angeles, CA
Society for Neuroscience 2007
November 3rd - 7th
San Diego, CA
American Heart Association 2007
November 3rd - 7th
Orlando, FL
Missouri Community College Association Annual Meeting
November 7th - 9th
Branson, MO
Front Range Neuroscience Group Annual Meeting
November 12th
Fort Collins, CO
National Association of Biology Teachers 2007
November 28th - December 1st
Atlanta, GA
Support and Symposia
Simultaneous Recording using Two PowerLabs on Chart 5 for Windows
Chart 5 may be used to synchronize the input from multiple PowerLabs. In order to perform this function two PowerLabs, two USB Cables, and one BNC-BNC cable are needed.
Step 1: Verify that each PowerLab unit is recognized by Windows individually; then, plug both PowerLab units into the computer and turn them on.
Step 2: Launch Chart 5 for Windows software. A dialog box will appear so that you can choose which PowerLab to run first. Select the PowerLab at the top of the list; this will be known as PowerLab #1.
Step 3: Access the File menu and select “New”. In the New Document dialog box, select the appropriate option for settings and make sure the radio button next to “Close ‘Document 1’ after creating the new document” is unchecked.
Step 4: With second Chart document active, access the Setup menu and click “Select PowerLab…”. Choose the second PowerLab unit in the list and click OK; this will be known as PowerLab #2.
Step 5: Make the Chart View window for PowerLab #1 active, access the Setup menu, select “Trigger…”, and enter the following settings:
Step 6: Make the Chart View window for PowerLab #2 active, access the Setupmenu, select “Stimulator…”, and enter the following settings:
Step 7: Connect a BNC-BNC cable from Output 1 of PowerLab #2 to Trigger on PowerLab #1.
Step 8: Click “Start” on the document that is linked to PowerLab #1; this will initiate the trigger for PowerLab #1.
Step 9: Start recording on the document that is linked to PowerLab #2. When you click “Start”, the stimulus voltage from PowerLab #2 will be sent to the trigger of PowerLab #1 and both documents will begin recording simultaneously.
If you have any further questions call technical support at 1-888-965-6040.
Recent Citations
Chronic intermittent hypoxis impairs baroreflex control of heart rate but enhances heart rate responses to vagal efferent stimulation in anesthetized mice
Min Lin, Rugao Liu, David Gozal, William B. Wead, Mark W. Chapleau, Robert Wurster, and Zixi (Jack) Cheng
AJP: Heart. 2007; 293(2): p. H997-H1006
Decreased age-related cardiac dysfunction, myocardial nitrative stress, inflammatory gene expression, and apoptosis in mice lacking fatty acid amide hydrolase
Sandor Batkai, Mohanraj Rajesh, Partha Mukhopadhyay, Gyorgy Hasko, Lucas Liaudet, Benjamin F. Cravatt, Anna Csiszar, Zoltan Ungvari, and Pal Pacher
AJP: Heart. 2007; 293(2): p. H909-H918
Visceral pain decreases tolerance to blood loss in conscious female but not male rabbits
Heidi L. Shafford and James C. Schadt
AJP: Regu. 2007; 293(2): p. R721-R728
Effects of 5-Cyclopropyl-2-[1-(2-fluoro-benzyl)-1H-pyrazolo[3,4-b]pyridine-3-yl]pyrimid
in-4-ylamine (BAY 41-2272) on Smooth Muscle Tone, Soluble Guanylyl Cyclase Activity, and
NADPH Oxidase Activity/Expression in Corpus Cavernosum from Wild-Type, Neuronal, and Endothelial Nitric-Oxide Synthase Null Mice
Cleber E. Teixeira, Fernanda B. M. Priviero, and R. Clinton Webb
J. Pharmacol. Exp. Ther. 2007; 322(3): p. 1093-1102
PowerLab Customers in North America
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