The Radnoti Langendorff Constant Pressure recirculating apparatus permits the researcher to create a constant pressure re-circulating Langendorff preparation for Mouse and Rat. With additional equipment left ventricular pressure, apical force using the built-in pulley system and coronary resistance can be measured.
Application
This type of system is often used for dose response studies in pharmacology. This constant pressure Langendorff system uses a peristaltic pump to perfuse the heart and since pressure is held constant, changes in coronary resistance are detected as changes in flow. By using multiple reservoirs or a syringe pump, different solutions or drug concentrations can be delivered to the heart and washed out.
The system uses a water-jacketed reservoir to maintain and gas the perfusion solution or buffer. A peristaltic pump draws from the reservoir and drives the solution up to a water-jacketed bubble trap via a water jacketed flex tube assembly, where bubbles are removed from the flow, and then down to the aortic cannula into the heart chamber via another water jacketed flex tube assembly. Radnoti water jacketed flex tube assemblies use a teflon inner tubing to prevent degassing of the perfusate during low flow experiments. Effluent from the heart is directed to waste or directed to the Membrane Oxygenator where gas is exchanged and perfusate can be routed back to the circulation loop for the heart.
As the system is configured for constant pressure, changes in resistance from the heart will result in flow rate variations that can be monitored via an accessory Flow-meter.
The Radnoti 130102EZ Constant Pressure re-circulating Mouse Heart system comes complete with Lab Stand Assembly, tubing pre-assembled, glassware and associated clamp hardware. Also included is the Radnoti 170051A Thermal Circulator Water Bath and a Peristaltic pump and pump head 170100-50, 170111. An accessory Tubing Connector Kit is also included for replacement connectors or minor variations to the system.
This Radnoti isolated perfused heart system for mouse can also be purchased as a complete data acquisition and analysis system in the Mouse Langendorff Constant Pressure recirculating System.
Ordering Information:
When ordering the 120103EZ apparatus please specify the mains power voltage.
Where V= 115 for 110-115 mains power and 220 for 220-240 mains power.
Note:
For countries with 115V power service, the Thermo circulator requires a 20 AMPS outlet
For countries with 220V power service, the Thermo circulator requires a 15 AMPS outlet.
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