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The Radnoti Porcine Working Heart System 140101EZ provides the researcher with a complete system to create both constant pressure and constant flow re-circulating and non-re-circulating Langendorff preparations. the system can also be sued in working heart preparations for Large Hearts with high flow rates such as Porcine.
This working heart system uses a peristaltic pump to generate the hydrostatic head for the constant pressure Langendorff preparation that initially sustains the heart. Perfusate is drawn from the High Volume 5-liter water jacketed reservoir, routed through the atrial bubble trap and diverted up to the aortic bubble trap at the atrial cannula. After the heart has stabilized, the direction of flow is changed via a set of tubing clamps at “Y” connectors acting as a 3-way valve at the atrial cannula to switch from retrograde to working heart mode by directing perfusate into the left atria under hydrostatic pressure (5 mm Hg nominal, adjustable). The perfusate then enters the left ventricle and is pumped by the heart against an adjustable pressure head set by the researcher. After load may be adjusted on the aortic line to vary resistance by incorporating the supplied compliance loop on the aortic side during working heart mode. Buffer may be directed to waste following the compliance loop or directed to the 140144 Large Volume Sheet Flow Oxygenating chamber for re-gassing and returned to the heart. At this point the main 5-liter High Flow buffer reservoir supply may be omitted from the circuit and a nominal fixed volume (set by the researcher) re-circulated through the system.
The benefit of a pump fed compliance chamber used to generate the constant pressure Langendorff is that there is no need to reposition elevated supply reservoirs. The heart chamber, components, and key perfusate lines are water-jacketed for superb temperature control.
Parameters measured with additional equipment:
* LVP
* ECG
* Perfusion Pressure
* Coronary Output Pressure
* Metabolism
* Coronary Flow
* Temperature
* Ion
Ordering Information:
When ordering the Working Heart Unit please specify the mains power voltage.
Where V= 115 for 110-115 mains power and 220 for 220-240 mains power.
Note:
For countries with 115V power service, the Thermo circulator requires a 20 AMPS outlet
For countries with 220V power service, the Thermo circulator requires a 15 AMPS outlet.
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