Intracellular Recording System

Intracellular Recording System

Overview:

The ML870B74-V Intracellular Recording System includes a high impedance electrometer for intracellular studies.

System Components

The system includes:

Accessories

Additional accessories which are purchased separately, are available for the IE-210-V Intracellular Electrometer Amplifier. They include:

Electrode Holders (selection based on the outer diameter of user’s glass electrode)

  • ESP/W-F10N E Series Electrode Holder (Str, Ag-AgCl Pellet & Wire, 1.0 mm)
  • ESP/W-F12N E Series Electrode Holder (Str, Ag-AgCl Pellet & Wire, 1.2 mm)
  • ESP/W-F20N E Series Electrode Holder (Str, Ag-AgCl Pellet & Wire, 2.0 mm)
  • ESP-F10N E Series Electrode Holder (Str, Ag-AgCl Pellet, 1.0 mm)
  • ESP-F12N E Series Electrode Holder (Str, Ag-AgCl Pellet, 1.2 mm)
  • ESP-F15N E Series Electrode Holder (Str, Ag-AgCl Pellet, 1.5 mm)
  • ESP-F20N E Series Electrode Holder (Str, Ag-AgCl Pellet, 2.0 mm)

IE-210-V Accessories

Note: Please specify line operating voltage (V = 115 or 220 VAC)

 

Features:

Intracellular Amplifier

  • High impedance microelectrode amplifier for intracellular studies
  • Low noise and low drift intracellular microelectrode amplifier
  • Internal 4-pole Bessel low pass filter
  • Fast response time for recording from high impedance fluid-filled glass electrodes
  • Simultaneous current injection stimulation and recording through a single electrode
  • Internal DC current injection up to ±100 nA in the Low range and up to ±1000 nA in the High range
  • Bridge Balance Output gains of x10, x20, and x50
  • High input impedance headstage (5 x 1011 Ω)
  • Capacitance Compensation for up to 50 pF
  • Lightweight and pencil-sized headstage mounts directly on any micromanipulator, allowing a direct connection to the headstage input
  • Driven headstage shield at the probe input permits attachment of additional shielding
  • Easy electrode resistance measurement using Electrode Test which produces a voltage proportional to the electrode resistance
  • Filter frequency is encoded by a stepped DC voltage at the Filter Telegraph BNC for input to a data acquisition system
  • Easily changeable between Ag wire or a Ag-AgCl pellet electrode holder

Data Acquisition System and Analysis

The PowerLab 8/30 is shipped with ADInstruments software (LabChart and Scope), associated manuals and cables. The system is also supplied with LabChart Pro software which includes all the ADInstruments LabChart Modules (excluding GLP). LabChart Modules provide application specific data acquisition, analysis and display features. The modules currently available include:

LabChart Pro entitles the purchaser to 5 years of FREE LabChart Pro software upgrades and updates. Purchasers are also entitled to any ADInstruments modules released during this 5 year period (excludes GLP software).

For more information see the LabChart Pro page or contact your nearest ADInstruments representative.


Applications:

This Intracellular Recording System includes a high impedance electrometer designed specifically for intracellular studies. The system can be used to measure voltage and/or current across the membrane of a cell. There are many intracellular recording techniques including:
  • Voltage Clamp – measures the ionic current across a cell's membrane when the membrane potential is held at a constant
  • Current Clamp - measures the membrane potential changes when injecting a constant current into a cell through the recording electrode.
  • Patch Clamp – measures voltage or current across ion channels using a blunt a micropipette
  • Sharp Electrode Technique – measures the potential inside the cell membrane with a sharp electrode (glass micropipette with a smaller pore)
For more information see the Intracellular Recordings, Neurophysiology, Patch & Voltage Clamping and Evoked Potentials applications pages.

Specifications:

Electrometer

  • Input Impedance: 5 x 1011 Ω shunted by 0.5 pF
  • Output Resistance: 100 Ω
  • Gain: x1 ± 0.1%
  • Probe Input Range: ±10 V
  • Risetime (10-90%): 25 µsec, measured through 20 MΩ
  • Noise Level: 25 µV p-p input shorted (0.1 Hz to 10 kHz) at x1 output & 250 µV p-p, 20 MΩ at input
  • Capacitance Compensation: 0-50 pF
  • Leakage Current: 1 pA typical, adjustable to zero
  • Input Offset: ± 200 mV, referred to input
  • Electrode Resistance Test: 1 mV/MΩ (Low Range) & 10 mV/MΩ (High Range)
  • Buzz: Amplitude variable 0 to 15 V
  • Frequency variable 100 Hz to 10 kHz

Current Injection Bridge

Bridge operates in Low and High current ranges.

  • Bridge Resistance Ranges: 0 to 100 MΩ & 0 to 1000 MΩ (Low Range) & 0 to 10 MΩ & 0 to 100 MΩ (High Range)
  • Internal DC Current Source: 0 to ± 100 nA (Low Range) & 0 to ± 1000 nA (High Range)
  • External Stimulus: Current is limited by electrode resistance up to maximums: ± 10 V ÷ electrode R or 0.5 µA (Low Range) & ± 10 V ÷ electrode R or 5 µA (High Range)
  • Max. Stimulus Input: ± 15 V
  • Stimulus Input Resistance: 10 KΩ
  • I Monitor Output: 100 mV/nA (Low Range) & 10 mV/nA (High Range)
  • Bridge Output Gain: x10 at front panel, x20 or x50 at rear panel
  • Bridge Output Filter: 4-pole low pass Bessel with cut-offs at 0.05, 0.1, 0.2, 0.5, 1, 2, 5, 10 and 20 kHz
  • Bridge Output Resistance: 100 Ω

Meter

  • Meter: 3-1/2 digit LED
  • Amplifier Output: 200 mV, 2 V and 20 V
  • Current: 200 nA and 2 µA
  • Power Requirements: 100 to 130 or 220 to 240 VAC, 50/60 Hz, 10 VA
  • Physical Dimensions: Probe: 12.5 mm D x 6.2 cm L with 1.8 m L cable & Case: 8.9 x 43.2 x 30.5 cm (H x W x D)
  • Shipping Weight: 5.5 kg
  • Warranty: Three years, parts and labor




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