Two Electrode Voltage Clamp Recording System

Overview:

The PL3508B75-V Two Electrode Voltage Clamp Recording System is designed for two-electrode, whole-cell voltage clamping of Xenopus Oocytes using the OC-725C-V Oocyte Clamp Amplifier. Two vented electrode holders with silver wires are typically required with the clamp; a straight type for use with the voltage headstage and a 45° type with mounting handle for use with current electrodes. Vents have been added to the electrodes to prevent pressure build-up inside the electrode which can damage Oocytes. Microelectrodes or glass electrodes/micropipettes are not supplied.

System Components:

• PL3508 PowerLab 8/35 (includes LabChart software)
• MLS260/7 LabChart Pro software
• PLA190 19 Inch Rack Adapter
• OC-725C-V Oocyte Clamp Amplifier, which includes:
  • 7250V Voltage Headstage for OC-725C-V
  • 7251I Bath Clamp Headstage for OC-725C-V
  • 7259C Current Electrode Cable for OC-725C-V
  • 725MC Oocyte Model Cell for OC-725C-V (includes rack mount hardware)
• ESW-F15V E Series Electrode Holder (Str, Vent, Ag Wire, 1.5 mm)
• E45W-F15VH E Series Electrode Holder (45°, Vent, Handle, Ag Wire, 1.5 mm)

Ordering Information:
When ordering the PL3508B75-V please specify the following.
Mains Power (V) = 115 for 110-115V or 220 for 220-240V

Accessories (purchased separately):

• Electrode Holders (selection based on the outer diameter of user’s glass electrode)
  • ESW-F10V E Series Electrode Holder (Str, Vent, Ag Wire, 1.0 mm)
  • ESW-F12V E Series Electrode Holder (Str, Vent, Ag Wire, 1.2 mm)
  • ESW-F20V E Series Electrode Holder (Str, Vent, Ag Wire, 2.0 mm)
  • E45W-F10VHE Series Electrode Holder (45°, Vent, Handle, Ag Wire, 1.0 mm)
  • E45W-F12VHE Series Electrode Holder (45°, Vent, Handle, Ag Wire, 1.2 mm)
  • E45W-F20VH E Series Electrode Holder (45°, Vent, Handle, Ag Wire, 2.0 mm)

Data Acquisition and Analysis:
The PowerLab 8/35 is shipped with ADInstruments LabChart software that provides data recording integrity, display and analysis features. The research system is also supplied with LabChart Pro software, which includes all the ADInstruments LabChart Modules (excluding GLP Client and Server). LabChart Modules provide application specific data acquisition, analysis and display features. 

LabChart Pro also entitles the purchaser to 5 years of FREE LabChart Pro software upgrades and updates. Purchasers are also entitled to any LabChart Module released during this 5 year period (excludes GLP Client and Server).

For more information see the LabChart Pro page or contact your nearest ADInstruments representative.

Features:

• High compliance voltage (±180 V)
• Unique bath clamp circuitry
• Ideal for clamping large cells and cell structures, e.g. squid axons
• Extended current measuring range
• Decreased noise level and 4-pole Bessel filter
• An internal switch permits measurements of the current in series with the current electrode, instead of in the bath
• Optional differential voltage headstages
• Buzz controls (1 kHz square wave) for each electrode aid in penetration of cell membranes with a minimum of leakage
• Overload alarm(audible and visual) indicate when the compliance voltage is exceeded safeguarding the Oocyte and indicating that current records are subject to saturation
• DC Offsets for both voltage and current electrodes
• Electrode Test for both electrodes
• Capacity Compensation for the Vm voltage input

Applications:

The Two Electrode Voltage Clamp Recording System is designed for two-electrode, whole-cell voltage clamping of Xenopus Oocytes using the OC-725C-V Oocyte Clamp Amplifier.

For more information visit the Patch & Voltage Clamping application page.

Specifications: