The PC-ONE Patch/Whole Cell Clamp is designed for use in a wide range of Patch Clamp studies. As well as many of the hardware features the unit is cost-effective in that it accepts seven types of headstages to give you the exact headstage for your application/s.
Features:
- Easy to Use, Very Cost Effective
- Low Noise, High Speed
- Can do Patch or Whole Cell with optional Dual Range PC-ONE-60 Remote Switchable Headstage
- Built in Leakage Subtraction
- Offsets up to ±300 mV
- 3-1/2 Digit LCD Panel Meter
- Telegraphs for GAIN and FILTER
- Eight Output Gains
- Auto Zero with SEARCH and HOLD capability
- Two Value Test Generator for pipette and seal resistance
- Unfiltered and Filtered Im Outputs
- Five position 3-pole Low Pass Bessel Filter
- 10 Megohm Headstage for Large Whole Cells
- Popular 50:1 and 10:1 Command Inputs
- Voltage/Current or Zero Current Mode from External Control
Ordering Information: There are three options available for purchase according to the type of headstage and application selected:
Option 1:
PC-ONE/H-P: For Large, standard or small whole cell clamp as well as extracellular patch and voltammetry studies:This option when ordered includes the PC-ONE amplifier and a single headstage (customer to specify headstage H = 10, 20, 30, 40 or 70) as well as one pipette holder (customer to specify P: 120=
HB120 or 180=
HB180).
Please note: The PC-ONE is shipped with the PC-ONE-40 headstage as standard if no other headstage is specified.
Option 2:
PC-ONE-B/P: For lipid bilayer studies:This option when ordered includes the PC-ONE amplifier with a PC-ONE-50 Bilayer Headstage and one pipette holder. Order a PC-ONE-B/120 for a
HB120 or PC-ONE-B/180 for a
HB180 Glass Pipette Holder.
Option 3:
For remote control dual range whole cell and patch PC-ONE-D/P:This option when ordered includes the PC-ONE amplifier with a PC-ONE-60 Dual Range Headstage and one pipette holder. Order a PC-ONE-B/120 for a
HB120 or PC-ONE-B/180 for a
HB180 Glass Pipette Holder.
The PC-ONE accepts SEVEN types of Headstages to give you the EXACT Headstage for your Application:The following table provides a guide for selecting the appropriate headstage for your application, for further information or assistance please contact your nearest
ADInstruments representative.
| Headstages |
Feedback Resistance
|
Current Range
|
Application |
| PC-ONE-10 |
10 Megohm
|
1000 nA
|
Large whole cell
|
PC-ONE-20
|
100 Megohm
|
100 nA
|
Standard whole cell
|
| PC-ONE-30 |
1 Gigohm
|
10 nA
|
Small whole cell
|
| PC-ONE-40 |
10 Gigohm
|
1 nA
|
Extracellular patch (also called
single channel or cell attached)
|
| PC-ONE-50 |
10 Gigohm/shorted
|
1 nA/infinity
|
Bilayer (Quick Charge)
|
| PC-ONE-60 |
100 Megohm/10 Gigohm
|
100/1 nA
|
Remote control dual range
whole cell and patch
|
PC-ONE-70
|
100 Megohm
|
100 nA
|
Voltammetry (2V command)
|
Application features with the select headstage include:
- Cell Attached (Single Channel) Patch Clamping: The 10 Gigohm standard Headstage (PC-ONE-40) or the optional Dual Range Headstage (PC-ONE-60) allow for low noise (0.30 pA rms) recording. Ease of use was paramount with the PC-ONE design team. The PC-ONE is equipped with the standard "Auto Zero" or HOLD Junction Potential Correction as well as a continuous SEARCH capability. The FILTER has five ranges suitable for all modes of operation. An optional 4-pole Bessel filter is also available (Model FL-4).
- Planar Lipid Bilayer Recording: A special Bilayer headstage (10 Gigohm feedback resistor) not only allows applications to 500pF Bilayers, but it also allows you to "short" the Bilayer during the onset of a step command to "quick charge" the Bilayer capacitance. A unique 10 Gigohm (Bilayer) position on the Headstage Selector switch modifies the Capacitance Compensation from the normal 10pF Patch application to the 500pF Bilayer application.
- Easy Computer Interfacing: The PC-ONE rear panel has a 15-pin connector that easily unplugs, breaking Input/Output connections (optional I/O cable). Thus if desired Im, FILTERED Im, Vm and Vtot can be disconnected very conveniently. The rear panel also contains the headstage connector, chassis/circuit ground jacks, Buz-1, Speed Test Input BNC, Test SYNCBNC, Gain and Filter Telegraph BNC's, and the power cord interlock
- Voltammetry (Electrochemical) Applications: A special headstage with a +/-2 volt command range allows the PC-ONE to "voltage clamp" a carbon-fiber electrode so that extracellular concentrations of various neurotransmitters can be measured at any specified oxidation/reduction potential. Headstages can be specified with 10 Megohm, 100 Megohm, 1 Gigohm or 10 Gigohm feedback sensing resistors (i.e., 1000nA down to 1nA full scale sensitivities).
- Easy Mode Control: Selectable Voltage Clamping and both Current Clamping with Command or Zero Command (protects cells when changing modes). A connector on the rear panel allows external computer control of modes. Two LED's indicate the mode selected.
- Whole Cell Voltage and Current Clamping: The PC-ONE handles three ranges of whole cell headstages for currents up to 1000nA (10 Megohm feedback). Standard LEAKAGE subtraction/compensation allows you to quickly adjust for leak errors on-line during an experiment.
Accessories:
- HB120 Pipette Holder for use with 1.0 to 1.2mm (OD) glass
- HB180 Pipette Holder for use with 1.5 to 1.8mm (OD) glass
- BUZ-1 Universal Buzz Box
Other Patch Clamps:
- BVC-700A (high speed current clamp, V clamp mode for seal test)
Warning: This equipment is not intended for use in human applications or human experimentation.