MPVS-Ultra Foundation System

Overview:

The PL3516B49 Mikro-Tip Pressure Volume System (MPVS)-Ultra Foundation System is configured for measurement of left ventricular pressure (LVP) and volume in small (rat or mouse) through to large animal (sheep, pig) hearts, using the appropriate pressure-volume (PV) catheter. A variety of PV catheters are available for particular species with all catheters having pressure sensors (single or dual) and multiple electrodes (see below). Due to their small size the sensors can be directly placed into the left ventricle of the heart for simultaneous measurement of pressure and volume with minimal disruption to heart function. In vivo, the catheters can be used in either closed or open-chest preparations as well as being used in the isolated working heart preparation.

The system includes a PowerLab, LabChart and LabChart Pro acquisition and analysis software. The analysis software includes the ADInstruments ECG Analysis and Blood Pressure Modules which are specifically configured to analyze and report pressure (ventricular or blood) parameters as well as examine any recordings of ECG.

Please Note:
Pressure-Volume catheters for Dog/Pig/Sheep, Rabbits/Cats, Mice/Rats are to be purchased separately. For more information or assistance please contact your nearest ADInstruments representative.

System Components:

• PL3516 PowerLab 16/35 (includes LabChart software)
• MLS260/7 LabChart Pro software
  • MLS375/7 PV Loop Module software
• 880-0168 MPVS-Ultra Pressure-Volume Unit (large and small animals)
• 910-1060 Rho Calibration Cuvette Kit
• 880-0169 MPVS-Ultra Cable Pack (10ft) (required for connecting PV catheters to the MPVS-Ultra Pressure-Volume Unit).
• 880-0172 MPVS-Ultra BNC Cable Pack (required for connecting the MPVS-Ultra Pressure-Volume Unit to the PowerLab).  Includes:
  • BNC-BNC 9" cables (10)
  • BNC-BNC 3' cables (2)
  • BNC-BNC 1' cable (1)

Mikro-Tip Pressure-Volume Catheters (purchased separately):
Multi Segment PV Catheters (dog/pig/sheep):

• VENTRI-CATH-507 Ventri-Cath Catheter (5F, 12E, 7mm, DField, Pigtail, 122cm)
• VENTRI-CATH-507S Ventri-Cath Catheter (5F, 12E, 7mm, DField, Straight, 122cm)
• VENTRI-CATH-510 Ventri-Cath Catheter (5F, 12E, 10mm, DField, Pigtail, 122cm)
• VENTRI-CATH-510S Ventri-Cath Catheter (5F, 12E, 10mm, DField, Straight, 122cm)
• VENTRI-CATH-512 Ventri-Cath Catheter (5F, 12E, 12mm, DField, Pigtail, 122cm)
• VENTRI-CATH-512S Ventri-Cath Catheter (5F, 12E, 12mm, DField, Straight, 122cm)
• VENTRI-CATH-515 Ventri-Cath Catheter (5F, 12E, 15mm, DField, Pigtail, 122cm)
• VENTRI-CATH-515S Ventri-Cath Catheter (5F, 12E, 15mm, DField, Straight, 122cm)
• SPR-562-1 PV Catheter (6F, 2P, 12E, 7mm, DField, Pigtail, 125cm, PU, Non Repairable)

Note: VENTRI-CATH catheter series require the additional purchase of CEC-10PV PV Extension Cable (Ventri-Cath to MPVS Ultra, 10ft) to connect to the 880-0168 MPVS-Ultra Pressure-Volume Unit (large and small animals).

Multi Segment PV Catheters (rabbits/cats):

• SPR-877 PV Catheter (3F, 10E, 2.5mm, DField, 120cm)
• SPR-889 PV Catheter (3F, 10E, 3mm, SField, U-tip, 80cm)
• SPR-894 PV Catheter (3F, 10E, 4mm, DField, U-tip, 80cm)

Single Segment PV Catheters (mice/rats):

• PVR-1030 Mouse PV Catheter (1F, 4E, 3.0mm, 4.5cm, PI, Non Repairable)
• PVR-1035 Mouse PV Catheter (1F, 4E, 3.5mm, 4.5cm, PI, Non Repairable)
• PVR-1045 Mouse PV Catheter (1F, 4E, 4.5mm, 4.5cm, PI, Non Repairable)
• SPR-839 Mouse PV Catheter (1.4F, 4E, 4.5mm, 4.5cm, PI)
• SPR-853 Mouse PV Catheter (1.4F, 4E, 4mm, Taper)
• SPR-847 Rat PV Catheter (1.4F, 4E, 9mm, 15cm, PI)
• SPR-869 Rat PV Catheter (2F, 4E, 6mm, 15cm, PI)
• SPR-838 Rat PV Catheter (2F, 4E, 9mm, 15cm, PI)
• SPR-878 Rat PV Catheter (2F, 4E, 12mm, 15cm, PI)
• SPR-858 Rat PV Catheter (2F, 4E, 14mm, 15cm, PI)

• SPR-848 Mouse PV Catheter (1.4F, 2P, 4E, 4.5mm, Apical)
• SPR-864 Mouse PV Catheter (1.4F, 2P, 4E, 4.5mm, Carotid)
• SPR-901 Rat PV Catheter (2F, 2P, 4E, 9mm, 15cm, Carotid)
• SPR-902 Rat PV Catheter (2F, 2P, 4E, 9mm, 15cm, Apical)

Available as a separate item, the MLA1052 Pressure Gauge is a kit, which provides the necessary items to calibrate the pressure transducer.

Data Acquisition and Analysis:
The PowerLab 16/35 is shipped with ADInstruments LabChart software that provides data recording integrity, display and analysis features. The research system is also supplied with LabChart Pro software, which includes all the ADInstruments LabChart Modules (excluding GLP Client and Server). LabChart Modules provide application specific data acquisition, analysis and display features. 

LabChart Pro also entitles the purchaser to 5 years of FREE LabChart Pro software upgrades and updates. Purchasers are also entitled to any LabChart Module released during this 5 year period (excludes GLP Client and Server).

For more information see the LabChart Pro page or contact your nearest ADInstruments representative.

Features:

The MPVS-Ultra Pressure-Volume Unit provides analog outputs for two pressure, a composite volume and a maximum of seven volume segments. The combined pressure and volume signals can be plotted in real time using LabChart to generate pressure-volume loops which are used to determine cardiac function.

The PowerLab 16/35 shipped with ADInstruments LabChart software provides data recording integrity, display and analysis features that include:

• Simultaneous recording and display up to 16 channels of data online
• Simultaneous recording and signal analysis
• Two independent stimulator outputs
• Wide range of low-pass filters
• AC or DC coupling
• A variety of measurements (with the appropriate Signal Conditioners and Transducers) including
  • Left ventricular pressure (LVP)
  • Left ventricular volume
  • Heart rate
  • Rate of pressure change (dP/dt)
  • Rate of volume change (dV/dt)
  • Systolic and end-diastolic pressure
  • ECG
  • Temperature

The research system is also supplied with LabChart Pro software, which includes all the LabChart Modules, such as the ECG Analysis Module, Blood Pressure Module and Peak Analysis Module for Windows which are specifically configured to analyze and report pressure (ventricular or blood) parameters as well as examine any recordings of ECG.

The PV Loop Module records and analyzes left ventricular pressure and volume data for hemodynamic research in animals. Analyses can be conducted during or after sampling. The module allows conversion of data to absolute volumes using cuvette calibration and correcting for parallel conductance using saline calibration - all in one data file. It also provides the ability to calculate many specific hemodynamic parameters including:

• Heart rate
• Maximum and minimum volume
• End-systolic volume
• End-diastolic volume
• Maximum and minimum pressure
• End-systolic pressure
• End-diastolic pressure
• Arterial elastance
• Cardiac output
• Stroke work
• Stroke volume
• Ejection fraction
• Maximum and minimum dP/dt
• Maximum and minimum dV/dt
• Pressure at dP/dt Max and dV/dt Max
• Volume at dP/dt Max and dP/dt Min ……and many more

For more information see the LabChart Pro page or contact your nearest ADInstruments representative.

In additon, the PVAN software extension (free to download) allows export of LabChart data in a selection directly into Millar Instruments's PVAN, MPress and PVAN Ultra software (purchased separately) applications.

Applications:

The MPVS-Ultra Foundation System is configured for measurement of left ventricular pressure (LVP) and volume in small (rat or mouse) through to large animal (sheep, pig) hearts, using the appropriate pressure-volume (PV) catheter. The system allows for the analysis of cardiac and cardiovascular parameters in models of cardiac hypertrophy, cardiac failure, cardiovascular remodeling and vascular occlusions, in addition to studies involving pharmacology/toxicology and transgenic manipulation.

For more information about left ventricular pressure-volume measurements using the MPVS-Ultra Foundation System visit the Ventricular Pressure-Volume application page.

Specifications: