Radnoti Working Heart Apparatus for Mice

Overview:

This 13010EZ-V Radnoti isolated perfused heart apparatus is ideal for preparations of working heart, constant flow and constant pressure Langendorff (recirculating and non-recirculating) preparations in mice.

The reservoir, heart chamber, bubble traps and interconnecting tubes are water-jacketed to maintain constant temperature, with the water temperature and flow controlled by the thermo/bath circulating pump.

The system includes a 2 L reservoir with an oxygenating bubbler attached. A peristaltic pump drives the perfusate through a membrane oxygenator to the High-Tech Heart Chamber. A series of compliance chambers/bubble traps are included to ensure that air bubbles are removed from the perfusion system.

The high-tech heart chamber has a threaded cap to ensure that the system temperature is maintained and includes access ports for stainless steel aortic and atrial cannulae, pacing electrodes and additional instrumentation. The chamber also includes a removable pulley to allow apical force measurements with an appropriate force transducer.

This Radnoti isolated perfused heart system for mice can also be purchased as a complete research system in the Radnoti Working Heart System for Mice.

Ordering Information:
When ordering the Working Heart Unit please specify the mains power voltage.
Where V= 115 for 110-115 mains power and 220 for 220-240 mains power.

Note:
For countries with 115V power service, the Thermo circulator requires a 20 AMPS outlet
For countries with 220V power service, the Thermo circulator requires a 15 AMPS outlet.

Options:
An optional system which has only the glassware and NO pumps please order the 130101EZ-NP.

Features:

• One system for both Langendorff and Working Heart preparations
  
• Double lumen water-jacketing insulated tubing to maintain solution temperature
• Single-pass perfusion or recirculation modes
• Modular construction for easy replacement of parts or updating of modified components
• Threaded cap on High-Tech Heart Chamber minimizes temperature loss and supports cannulae
• Color-coded system for quick setup
• Incorporates Quick Disconnect fittings

Applications:

The Radnoti Working Heart Apparatus for Mice allows the heart to pump fluid via the normal left ventricular circulatory pathway as the perfusate enters the heart via the cannulated left atrium, passes through to the left ventricle and is ejected out of the aorta.

Parameters Measured in a Working Heart Preparation:

• Atrial Inflow and Aortic Outflow if appropriate flow measurement devices are used (i.e. transonic flow probes)*
• Coronary Flow (Atrial Inflow minus Aortic Outflow)*
• Left-Atrial Pressure or Volume* (Preload)
• Left-Ventricular Pressure. Left ventricular volume may also be simultaneously recorded using a Millar Mikro-Tip Pressure-Volume conductance catheter
• Aortic Pressure (Afterload)
• Cardiac contractility with an appropriate force transducer connected to the apex
• Systolic & Diastolic Pressure from the aortic line
• Left ventricular dP/dt
• HR
• ECG with suitable electrodes*
• Stroke Volume and Cardiac Output may also be recorded provided an accurate flow measurement is obtained*

* Additional pressure transducers or flow probes are required to measure these parameters.

For more information see the Working Heart application page.

Specifications: