Confocal Wire Myograph

Overview:

The DMT120CW Confocal Wire Myograph is specifically designed for obtaining highly resolved images of fluorescent dyes within living tissues (> 60 µm) using laser-scanning confocal microscopy (LSCM). In combination with LSCM, simultaneous measurements of isometric forces and intracellular Calcium or pH can be made. The DMT120CW includes a control unit with temperature control, built-in high-resolution force transducer and micrometer.

The control unit has analog output which can be connected directly to a PowerLab BNC input. The Confocal Wire Myograph System is also available and includes a PowerLab data acquisition system, LabChart Pro and DMT120CW. Optical components are NOT included.

When purchasing the DMT120CW the following items are included:

Myo- Interface Unit

• Myograph connection cable with a temperature probe
• Power cord (The shape of the AC plug varies by country; be sure that the plug has the right shape for your location)

Confocal Wire Myograph Unit

• Calibration kit (including bridge , balance and 2 gram weight)
• Chamber cover
• Chamber volume 10 ml

Accessories

• 1 roll of 40 μm stainless steel wire
• 1 tube of high vacuum grease
• 1 tube of grease for linear slides
• 4 spare screws for mounting of jaws
• 10 extra myograph window glasses
• 2 Allen keys
• 1 small screwdriver
• 40 mm funnel

Manuals

• User manual for the Confocal Wire Myograph System Model 120CW
• Procedures for investigation of small vessels using small vessel myograph, by Professor M. J. Mulvany, Department of Pharmacology, Aarhus University,Denmark

Warranty Exclusion

• DMT force and pressure transducers, either separately or part of myograph systems manufactured by DMT A/S, are disclaimed from any warranty

Features:

• Single channel wire myograph (10ml)
• Bath design allows easy access for high numerical aperture lenses used in standard upright confocal microscopy
• Reverse mounting supports allow vertical positioning of the isolated blood vessel

Applications:

The DMT120CW Confocal Wire Myograph is specifically designed for obtaining highly resolved images of fluorescent dyes within living tissues using laser-scanning confocal microscopy. It is ideal for physiological and pharmacological investigations using arterial microvessels, and a variety of other tissue types such as small veins, renal arcuate arteries, bronchi and ureter. The technique, is suited to vessels with internal diameters > 60 μm.

Parameters which can be measured include:

• Normalized internal diameter
• Measuring tissue wall thickness
• Isometric (force) responses to drugs (agonists/antagonists)
• Simultaneous measurements of isometric response and cell membrane potential
• Simultaneous measurements of isometric responses and ion concentrations inside the cell
• Analysis of tissue fixed under clearly defined conditions for microscope measurements
• Fluorescent Microscopy; Intracellular [Ca²⁺] or pH.
  

For more information see the Wire Myography application page.

Specifications:

Technical Specifications 310A 410A 510A 610M 120CW Tissue/Vessel size >60 μm >60 μm >60 μm >60 μm / >450 μm up to 10 mm >60 μm Chamber(s) Single bath Dual, independent or common Dual, independent or common Four individual chambers Single bath/conical shape Chamber material Acid-resistant stainless steel Acid-resistant stainless steel Acid-resistant stainless steel Acid-resistant stainless steel Acid-resistant stainless steel Chamber volume Max. 10 ml Max. 10 ml Max. 10 ml Max. 8 ml Max. 10 ml Chamber suction No No No Manual or automatic, time controlled, user defined No Chamber cover Yes With connection for suction and gassing With connection for suction and gassing Supplied with connections for gassing With connection for suction and gassing Force range -100 -/+200 mN -100 - /+200 mN -100 - /+200 mN User selectable at +/-200/400/800/1600 mN -100 - /+200 mN Force resolution 0.01mN 0.01mN 0.01mN 0.01mN 0.01mN Micropositioners Manually operated Manually operated Automatical linear motor operated Manually operated Manually operated precision micrometer Weight calibration Manual Manual Manual Semi Automatic Manual Heating Built into chamber, independent of superfusion Built into chamber, independent of superfusion Built into chamber, independent of superfusion Built into chamber, independent of superfusion Built into chamber, independent of superfusion Temp. range Ambient temp.  to 50 °C Ambient temp.  to 50 °C Ambient temp.  to 50 °C Ambient temp.  to 50 °C Ambient temp.  to 50 °C Temp. resolution 0.1 °C 0.1 °C 0.1 °C 0.1 °C 0.1 °C Temp. probe External External External External External Output reading Force (mN) Force (mN) Force (mN) Force (mN) Force (mN) Analog output Up to four output, 1.0V full scale for all acquired signals, user defined Up to four output, 1.0V full scale for all acquired signals, user defined Up to four output, 1.0V full scale for all acquired signals, user defined Independent filtered 4 channel output at 1.0V full scale Up to four output, 1.0V full scale for all acquired signals, user defined Serial output Serial interface - RS232/RS485 Serial interface - RS232/RS485 Serial interface - RS232/RS485 Serial interface - RS232/RS485 Serial interface - RS232/RS485 Voltage 100 to 240 VAC (auto) 50/60Hz 100 to 240 VAC (auto) 50/60Hz 100 to 240 VAC (auto) 50/60Hz 100 to 240 VAC (auto) 50/60Hz 100 to 240 VAC (auto) 50/60Hz Ambient temp. 15-30 °C 15-30 °C 15-30 °C 15-30 °C 15-30 °C Optional accessories - range pH 0 - 14 pH 0 - 14 pH 0 - 14 pH 0 - 14 - temp. correction 0 - 50 °C 0 - 50 °C 0 - 50 °C 0 - 50 °C