Langendorff Apparatus & Thermostat Controller

Overview:

The ML176-V Langendorff Apparatus Unit is a self-contained and thermostatically controlled unit for the perfusion of small animal hearts. It contains two separate perfusate reservoirs that are constantly oxygenated and kept at a constant bath temperature. When used as part of the Langendorff System, the solutions pass through tubes around the Peristaltic Pump (regulated by the STH Pump Controller) in order to pressurize them.

The pressurized perfusate fluids are subsequently warmed when they re-enter the bath unit in coiled tubes and feed into the heated junction block,from which the cannulated heart is attached. Perfusate is directed towards the junction block, however only one is used at a time to perfuse the heart with the other redirected via the 3-way valve back to the reservoir. This allows an easy change between the two reservoirs and prevents the formation of air bubbles or entry of air into the heart upon reservoir change. The heated junction block ensures the perfusate is maintained at a constant temperature prior to entering the heart.

The system is best suited for use with small animal hearts such as mouse, rat and guinea pig. For a complete research system visit our Langendorff System page.

For isolated hearts using larger animals such rabbits we recommend the Working Heart System for Rats/Rabbits.

Ordering Information:
When ordering the ML176, please specify the mains power voltage.
Where V= 115 for 110-115 mains power and 220 for 220-240 mains power.

Features:

• A self-contained and thermostatically controlled apparatus
• Two separate perfusate reservoirs that are constantly oxygenated and kept at a constant bath temperature
• A heated junction block to ensure the perfusate is maintained at a constant temperature prior to entering the heart

Applications:

The Langendorff Apparatus with Thermostat Controller is best suited for use with small animal hearts such as mouse, rat and guinea pig. The aorta is cannulated and the solution is delivered in a retrograde (backward) fashion down the aorta either at a constant flow rate or at a constant pressure. The perfusate does not enter the heart chambers themselves but flows through the coronary vascular system, thereby supplying the heart muscle with the nutrients required for normal function.

Parameters Measured in a Langendorff Preparation (additional equipment required):

• Perfusion Pressure
• Perfusion Flow
• Left Ventricular Pressure (LVP)/Developed Pressure/Systolic & Diastolic Pressures
• ECG/EKG and Monophasic Action Potentials
• Temperature

For more information see the Langendorff Heart application page.

Specifications: