MPVS-Ultra Foundation System

The MPVS-Ultra Foundation System is configured for measurement of left ventricular pressure (LVP) and volume in small (rat or mouse) through to large animal (sheep, pig) hearts, using the appropriate pressure-volume (PV) catheter. A variety of PV catheters are available for particular species with all catheters having pressure sensors (single or double) and multiple electrodes (see below). Due to their small size the sensors can be directly placed into the left ventricle of the heart for simultaneous measurement of pressure and volume with minimal disruption to heart function. In vivo, the catheters can be used in either closed or open-chest preparations as well as being used in the isolated working heart preparation.

The system includes a PowerLab, LabChart and LabChart Pro acquisition and analysis software. The analysis software includes the ADInstruments ECG Analysis and Blood Pressure Modules which are specifically configured to analyze and report pressure (ventricular or blood) parameters as well as examine any recordings of ECG.

Please Note:
Pressure-Volume catheters for Dog/Pig/Sheep, Rabbits/Cats, Mice/Rats are to be purchased separately. For more information or assistance please contact your nearest ADInstruments representative.

System Components:

• ML880 PowerLab 16/30, 16 Channel Recorder
• MLS260 LabChart Pro  (Win & Mac)
• 880-0168 MPVS-Ultra Pressure-Volume Unit (large and small animals)
  
• 910-1060 Rho Calibration Cuvette kit
• 880-0169 MPVS-Ultra Cable pack (10ft) (required for connecting PV catheters to the MPVS-Ultra Pressure-Volume Unit) Includes:
• 1x CEC-10E PV Extension Cable (10') for pressure and volume measurement and
• 1x PEC-10D Pressure Extension Cable (10') for 2nd sensor pressure measurement

• 880-0172 MPVS-Ultra BNC Cable pack (required for connecting the MPVS-Ultra Pressure-Volume Unit to the PowerLab).  Includes:
• BNC-BNC 9" cables (10)
• BNC-BNC 3' cables (2)
• BNC-BNC 1' cable (1)
• 001-1056 PVAN Ultra Analysis Software

Suitable Pressure-Volume Catheters include:
Multi Segment PV Catheters (dog/pig/sheep)

• SPR-550-1 Multi-Seg PV Catheter (5F, 12E D-Field 7mm, Straight, 125cm)
• SPR-550-2 Multi-Seg PV Catheter (5F, 12E D-Field 9mm, Pigtail, 125cm)
• SPR-550-5 Multi-Seg PV Catheter (5F, 12E D-Field 7mm, Pigtail, 125cm)
• SPR-550-7 Multi-Seg PV Catheter (5F, 12E D-Field 10mm, Pigtail, 125cm)
• SPR-550-8 Multi-Seg PV Catheter (5F, 12E S-Field 7mm, Pigtail, 125cm)
• SPR-562-1 Multi-Seg PV Catheter (6F, 2P, 12E D-Field 7mm, 125cm)

Multi Segment PV Catheters (rabbits/cats)

• SPR-845 Multi-Seg PV Catheter (3F, 8E S-Field 3mm, U-tip, 120cm)
• SPR-855 Multi-Seg PV Catheter (3F, 10E D-Field 4mm, Straight, 120cm)
• SPR-856 Multi-Seg PV Catheter (3F, 10E S-Field 4.5mm, Straight, 120cm)
• SPR-877 Multi-Seg PV Catheter (3F, 10E D-Field 2.5mm, Straight, 120cm)
• SPR-889 Multi-Seg PV Catheter (3F, 10E S-Field 3mm, U-tip, 80cm)
• SPR-894 Multi-Seg PV Catheter (3F, 10E D-Field 4mm, U-tip, 80cm)
• SSD-1033 Multi-Seg PV Catheter (3F, 8E S-Field 4.3mm, U-tip, 140cm)

Single Segment PV Catheters (mice/rats)

• PVR-1030  Mouse PV Catheter (1F, 4E, 3mm) - Non Repair
• PVR-1035  Mouse PV Catheter (1F, 4E, 3.5mm) - Non Repair
• PVR-1045  Mouse PV Catheter (1F, 4E, 4.5mm) - Non Repair
• SPR-819    Rat/Mouse Selectable Seg PV Catheter (1.4F, 6E, 9mm/14mm)
• SPR-866    Rat/Mouse Selectable Seg PV Catheter (1.4F, 6E, 4mmmm)
• SPR-839    Mouse PV Catheter (1.4, 4E, 4.5mm)
• SPR-847    Rat PV Catheter (1.4F, 4E, 9mm)
• SPR-869    Rat PV Catheter (2F, 4E, 6mm)
• SPR-838    Rat PV Catheter (2F, 4E, 9mm)
• SPR-878    Rat PV Catheter (2F, 4E, 12mm)
• SPR-858    Rat PV Catheter (2F, 4E, 14mm)
• SPR-851    Rat PV Catheter (3F, 4E, 20mm)
• SPR-853    Mouse Dual PV Catheter (1.4F, 2P, 4E, 4mm)
• SPR-848    Mouse Dual PV Catheter (1.4F, 2P, 4E,4.5mm, Ap)
• SPR-864    Mouse Dual PV Catheter (1.4F, 2P, 4E,4.5mm, Car)
• SPR-901    Rat Dual PV Catheter (2F, 2P, 12mm, 4E)
• SPR-902    Rat Dual PV Catheter (2F, 2P, 12mm, 4E 9mm Ap)

Available as a separate item, is a Pressure Gauge kit which provides the necessary items to calibrate the pressure transducer.

The 16-channel PowerLab 16/30 data acquisition system provides the ability to record LVP and volume as well as the opportunity to simultaneously measure other physiological parameters such as temperature and ECG with the appropriate Signal Conditioners and Transducers.

The MPVS-Ultra Pressure-Volume Unit provides analog outputs for two pressure, a composite volume and a maximum of seven volume segments. The combined pressure and volume signals can be plotted in real time using LabChart to generate pressure-volume loops which are used to determine cardiac function.

LabChart allows the user to simultaneously record and display up to 16 channels of data online. These may include left ventricular pressure, left ventricular volume, heart rate, rate of pressure change (dP/dt), rate of volume change (dV/dt), systolic and end-diastolic pressure. Signals, including pressure-volume plots, can be displayed and calculated online whilst recording. LabChart data files can be easily imported into PVAN software for additional offline analysis. The PVAN software provides the ability to calculate many specific hemodynamic parameters.

The MPVS-Ultra Foundation System allows for the analysis of cardiac and cardiovascular parameters in models of cardiac hypertrophy, cardiac failure, cardiovascular remodeling and vascular occlusions, in addition to studies involving pharmacology/toxicology and transgenic manipulation.

Some of the parameters which can be calculated using PVAN include:

• Heart rate
• Maximum and minimum volume
• End-systolic volume
• End-diastolic volume
• Maximum and minimum pressure
• End-systolic pressure
• End-diastolic pressure
• Arterial elastance
• Cardiac output
• Stroke work
• Stroke volume
• Ejection fraction
• Maximum and minimum dP/dt
• Maximum and minimum dV/dt
• Pressure at dP/dt Max and dV/dt Max
• Volume at dP/dt Max and dP/dt Min ……and many more

Data Acquisition and Analysis:
The PowerLab 16/30 is shipped with ADInstruments software (LabChart and Scope), associated manuals and cables. The ML880/P is also supplied with LabChart Pro software which includes all the ADInstruments LabChart Modules (excluding GLP). LabChart Modules provide application specific data acquisition, analysis and display features.

The modules currently available include:

• Dose Response (Windows) new
• Peak Analysis (Windows) new
• Blood Pressure (Windows)
• ECG Analysis (Windows)
• Heart Rate Variability (Windows & Macintosh)
• Spike Histogram (Windows & Macintosh)
• Metabolic (Windows & Macintosh)
• Video Capture (Macintosh)
• Cardiac Output (Windows)
• DMT Normalization (Windows & Macintosh)
  

In purchasing this system containing LabChart Pro entitles the purchaser to 5 years of FREE LabChart Pro software upgrades and updates. Purchasers are also entitled to any ADInstruments modules released during this 5 year period (excludes GLP software).

For more information see the LabChart Pro page or contact your nearest ADInstruments representative.