Patch Clamp Amplifier with 5101-10G Headstage
- Product# PC-501A/10-V
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Overview:
The PC-501A/10-V Patch Clamp Amplifier with 5101-10G(10 GΩ) Headstage is a workhorse patch clamp amplifier suitable for recording single channel currents up to ±1 nA in either research or teaching labs. The amplier is included in the PL3508B79/10-V Patch Clamp Recording System with 5101-10G Headstage.
PC-501A/10-V includes:
- PC501A Patch Clamp Amplifier
- 5101-10G Headstage for PC-501A/10-V (10 GOhm)
Voltammetry with the PC-501A:
Simple modifications to the PC-501A make it suitable for voltammetric measurements. They include increasing the holding voltage potential to ±1 V and the external voltage signal level to ±2 V maximum. Internal switches can be installed to allow switching between normal (patch) and voltammetry operation.
Note: Please specify V=115 or V=220 mains power
Alternate Headstages:
Other resistive-feedback headstages are also available for the PC-501A Patch Clamp Amplifier:
- 5101-01G Headstage for PC-501A/9-V (1 GOhm) for of whole cell studies with currents up to ±10 nA and is included in the PC-501A/9-V Patch Clamp with 5101-01G Headstage and in PL3508B79/9-V Patch Clamp Recording System with 5101-01G Headstage.
- 5101-100M Headstage for PC-501A/8-V (100 MOhm) for of whole cell studies with currents up to ±100 nA and is included in the PC-501A/8-V Patch Clamp with 5101-100M Headstage and in PL3508B79/8-V Patch Clamp Recording System with 5101-100M Headstage.
Features:
- Independent V-hold and I-hold circuitry
- Three current clamp response speeds increase stability of large membrane clamps
- 4-pole low-pass Bessel filter
- Internally generated test signals
- Variable duration zap circuit
Operating Modes:
V Clamp Mode: Input is clamped to a maximum of ±1 V. Commands are the sum of the holding voltage and any external input signals.
Zero Current Mode: Bridges the voltage and current clamp modes. All commands are inactive except the junction zero, which functions in this case as an offset control for the electrode and tip potentials associated with the pipette.
I Clamp Mode: In the current clamp mode, active commands are the current hold and any external command input signals, summed. Clamp stability is enhanced with the selection of three clamp speeds.
Compensation Controls:
Voltage Offsets (including junction potentials): Automatically compensated with the auto zero or manually with the junction zero.
Capacity Compensation: Paired controls; fast (0-5 µs), medium (0-2 ms) and slow (0-20 ms) compensate the current transient caused by a step command signal. Each pair has a separate control for amplitude and time constant.
Series Resistance: Signal errors contributed by the electrode (access) resistance in whole cell recording are subtracted from the output with the series R comp control. The compensated resistance (0-100 MΩ) is read from a ten turn dial.
Leak Subtract: Used to compensate leakage current due to the voltage drop across the seal (shunt) resistance.
Outputs/Signal Conditioning:
The membrane current signal Im is bandwidth limited by a 4-pole Bessel filter with six frequency cut-offs and can be bypassed to obtain the full 10 kHz bandwidth. Gain is selected with a 7 position switch and scaled to the headstage resistor.
Additional outputs are Vm x 10 (membrane voltage at x10 gain) active in current clamp mode and Vc x 10, the sum of all commands at x10 gain. Rear panel outputs include; gain telegraph, a DC signal which communicates to a data acquisition system the Im output gain level and the headstage in use, and sync output, a signal to synchronize an oscilloscope or computer with one of the internal test signals.
