The following headstages are for use with the PC-ONE
Whole Cell Patch Clamp. The following table provides a guide for selecting the appropriate headstage for your application, for further information or assistance please contact your nearest
ADInstruments representative.
| Headstages |
Feedback Resistance
|
Current Range
|
Application |
| PC-ONE-10 |
10 Megohm
|
1000 nA
|
Large whole cell
|
PC-ONE-20
|
100 Megohm
|
100 nA
|
Standard whole cell
|
| PC-ONE-30 |
1 Gigohm
|
10 nA
|
Small whole cell
|
| PC-ONE-40 |
10 Gigohm
|
1 nA
|
Extracellular patch (also called
single channel or cell attached)
|
| PC-ONE-50 |
10 Gigohm/shorted
|
1 nA/infinity
|
Bilayer (Quick Charge)
|
| PC-ONE-60 |
100 Megohm/10 Gigohm
|
100/1 nA
|
Remote control dual range
whole cell and patch
|
PC-ONE-70
|
100 Megohm
|
100 nA
|
Voltammetry (2V command)
|
Application features with the select headstage include:
- Cell Attached (Single Channel) Patch Clamping: The 10 Gigohm standard Headstage (PC-ONE-40) or the optional Dual Range Headstage (PC-ONE-60) allow for low noise (0.30 pArms) recording. Ease of use was paramount with the PC-ONE design team. The PC-ONE is equipped with the standard "Auto Zero" or HOLD Junction Potential Correction as well as a continuous SEARCH capability. The FILTER has five ranges suitable for all modes of operation. An optional 4-pole Bessel filter is also available (Model FL-4).
- Planar Lipid Bilayer Recording: A special Bilayer headstage (10 Gigohm feedback resistor) not only allows applications to 500pF Bilayers, but it also allows you to "short" the Bilayer during the onset of a step command to "quick charge" the Bilayer capacitance. A unique 10 Gigohm (Bilayer) position on the Headstage Selector switch modifies the Capacitance Compensation from the normal 10pF Patch application to the 500pF Bilayer application.
- Easy Computer Interfacing: The PC-ONE rear panel has a 15-pin connector that easily unplugs, breaking Input/Output connections (optional I/O cable). Thus if desired Im, FILTERED Im, Vm and Vtot can be disconnected very conveniently. The rear panel also contains the headstage connector, chassis/circuit ground jacks, Buz-1, Speed Test Input BNC, Test SYNCBNC, Gain and Filter Telegraph BNC's, and the power cord interlock
- Voltammetry (Electrochemical) Applications: A special headstage with a +/-2 volt command range allows the PC-ONE to "voltage clamp"a carbon-fiber electrode so that extracellular concentrations of various neurotransmitters can be measured at any specified oxidation/reduction potential. Headstages can be specified with 10 Megohm, 100 Megohm, 1 Gigohm or 10 Gigohm feedback sensing resistors (i.e., 1000nA down to 1nA full scale sensitivities).
- Easy Mode Control: Selectable Voltage Clamping and both Current Clamping with Command or Zero Command (protects cells when changing modes). A connector on the rear panel allows external computer control of modes. Two LED's indicate the mode selected.
- Whole Cell Voltage and Current Clamping: The PC-ONE handles three ranges of whole cell headstages for currents up to 1000nA (10 Megohm feedback). Standard LEAKAGE subtraction/compensation allows you to quickly adjust for leak errors on-line during an experiment.
Accessories:
- HB120 Pipette Holder for use with 1.0 to 1.2mm (OD) glass
- HB180 Pipette Holder for use with 1.5 to 1.8mm (OD) glass
- BUZ-1 Universal Buzz Box
Warning: This equipment is not intended for use in human applications or human experimentation.