Dual Ussing Chamber w/Millicell Insert 12 mm

Overview:

The U2500/M is a Dual Ussing Chamber with Millicell Insert 12 mm.

The Self-Contained Ussing Chambers systems come in single-channel (U9926) and dual-channel (U2500) models and offer many advantages over the Classic design. Both systems make use of inserts to secure and position the tissue or culture cup within the chamber body.

Chamber bodies (single- and dual-chamber models) consist of chamber-halves which clamp around the inserts. Each chamber-half has four ports that enter into its respective bath compartment: a voltage electrode port, a current electrode port, an air/gas port, and a solution drain port. Solution warming is provided by pumping heated water through each chamber-half and there are no delicate glass heat exchangers to be broken. The dual-chamber U2500 has the additional feature of including four needle valves for adjusting the air/gas mixture into each individual chamber-half. Inserts can be easily exchanged between experiments. Solutions are removed from the chambers through the drain valves and the halves unclamped from each other. The insert is then simply replaced. Inserts are available to support a variety of tissue sizes and culture cups.

Included with all U2500 Dual Ussing Chambers: 

• 2x U9975A Ussing Electrode Set (2 Ag/AgCl pellets and 2 Ag wires)
• 2x U9565SC Ussing electrode bridge fitting kit (12 pkg 6 adapters)
• 2x U9406 Tubing Kit (Hose & Luer fittings) for Ussing Chambers

Standard Accessories Supplied with U9926 and U2500:

TheU9926 (single-channel chamber is supplied with 1 electrode set(consisting of 2 voltage and 2 current electrodes), 12 bridge fittings with adapters; and a tubing/ fitting kit. The U2500 (dual channel)chamber is supplied with two each of the above described accessories.Chamber inserts are ordered separately.

Accessories purchased separately:

• U9924M-12 Culture Cup w/Millicell Insert 12 mm
• U9605 O-rings for Ussing Chambers (4 pack)
• U9985 Bridge Fitting Adapters for Ussing Chambers (6 pack)

For use with:

• EC-825A-V Dual Channel Epithelial Voltage Clamp
• PL3508B78-V Dual Channel Epithelial Voltage Clamp System

Features:

• Modular design for separate inserts
• No glass circulation reservoirs
• Inserts are quickly exchanged between experiments
• Solution warming is provided by pumping heated water through the chamber halves

Applications:

The Dual Ussing Chamber w/Millicell Insert is a tool used to measure the short-circuit current, which is used as an indicator of net ion transport taking place across an epithelium. Ussing chambers are increasingly being used to measure ion transport in both native tissue and monolayer of cells grown on permeable supports.

For more information see the Ussing Chamber application page.

Specifications: