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lab protocols
Basic concepts & analysis focus
Curriculum based modules
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Using Lt, zoology and physiology students can investigate basic and applied concepts in neuro and muscle physiology. Courses are designed with common undergraduate insect, annelid, amphibian and mammalian preparations.
The Otago Polytechnic School of Nursing uses Lt to increase student engagement and improve pass rates.
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including pre-made systems and kits for your subject area.
Animal Metabolism
Students are introduced to indirect calorimetry using a Gas Analyzer and PowerLab to record metabolic variables. These are then used to determine the metabolic rate and the respiratory exchange ratio (RER), which is a good estimate of the respiratory quotient (RQ).
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Earthworm Smooth Muscle
Students investigate the responses of smooth muscle to neurotransmitters, temperature, and ions. The earthworm (Lumbricus terrestris) gut provides a robust smooth muscle preparation that can be mounted in an organ bath and attached to a force transducer. This arrangement will enable students to measure contraction rate and force.
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Frog Heart
Explore the basic principles of cardiac muscle physiology, including contraction force, electrocardiogram (ECG) and the effect of neurotransmitters on the heart. Students also apply the Frank-Starling law of the heart to explain the relationship between stretch of cardiac muscle and the force of contraction.
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Frog Skeletal Muscle
Students will learn about these properties of skeletal muscle: twitch recruitment, effects of muscle stretch, summation, tetanus, and fatigue. The experiment is carried out using an isolated frog gastrocnemius muscle.
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Gin Trap Closure Reflex
In this lab, learners will become familiar with administering tactile stimulation to Manduca sexta larvae and pupae. They record and analyze EMG data from a pupal preparation that they have set up themselves.
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Cockroach Sensory Nerve
Students record compound action potentials (CAPs) from a cockroach leg nerve in response to various physical stimuli. Students examine CAP amplitudes and frequency, and identify different “classes” of CAP that represent different individual axons.
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Cockroach Ventral Nerve Cord
Students record extracellular action potentials from the ventral nerve cord of a large cockroach, or similar insect. Spontaneous activity, and different types of stimuli, (including tapping the bench, clapping, air puffs, and touching), are compared and analyzed using frequency histograms. Students will also determine the nerve conduction velocity of the ventral nerve.
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Earthworm Action Potentials
Obtain extracellular recordings of action potentials from an anesthetized earthworm to examine the threshold potential, the all-or-none response, the refractory period, and the conduction velocity of the nerve.
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Frog Nerve
Students measure compound action potentials (CAPs) from an isolated frog sciatic nerve to explore the basic physiological properties of nerve impulses, including the threshold, refractory period, and conduction velocity.
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Frog Neuromuscular Junction
Students investigate the relationship between skeletal muscle and motor nerve supply. Using an isolated frog gastrocnemius and sciatic nerve students will explore twitch recruitment, muscle fatigue, and the effects of tubocurarine.
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Intracellular Action Potentials
Students make intracellular recordings from neurons in a leech ganglion. They will become familiar with an intracellular amplifier, and with the use for glass microelectrodes. Students will record and analyze both resting potentials and action potentials, of different neuronal cell types.
Please note, additional electrodes and electrode holders may be needed for this experiment.
This lesson requires one of the PowerLabs below
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Getting Started with Lt
In this module students learn how to record and analyze data in Lt. A "Pre-Lab" lesson shows students what to expect in Lt labs and lets them explore the types of signals they might record. They also practice their data analysis skills prior to the lab, where they record and analyze their own pulse.
Over 360 fully editable nursing, medicine and physiology lessons.
Create, customize and deliver your own interactive content that students can access anywhere.
Students can record and analyze physiological signals in Lt using PowerLab, to reinforce theory through experimentation.
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The ideal DAQ solution for life science classroom experiments. PowerLab supports engaging, hands-on learning with simple set-up and good quality data. Will handle repeated and heavy use by students and maintain data accuracy and reliability. Learn more »
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