Introduction


CrawFly January 2023 Workshop

 

Pomona College

Claremont, CA, United States

January 4th - 8th, 2023

 

Click here to register for this event

 

Course Overview

ADInstruments and the Department of Biology at Pomona College will co-host a 5 day hands-on workshop for higher education life science instructors seeking to expand their curriculum reach in the neurosciences.

The training event will have two major components. The first half of the workshop is based on topics explored in the "Crawdad Project," a program funded by the National Science Foundation to promote the use of invertebrates in undergraduate physiology and neuroscience lab courses. The second half explores the power of neurogenetics to teach the optogenetic control of animal behavior and nervous system physiology. In addition, workshop participants will learn to build Do-It-Yourself (DIY) lab classroom products such as micromanipulators, optogenetic light sources and inexpensive fluorescence microscopes.

 **2 Scholarships are available for this workshop - please see information below. The application deadline is November 28th, 2022.**

Workshop Agenda

CRAWDAD:

Topics to be covered:• Motor innervation • Neuroanatomy • Sensory systems • Ionic bases of resting and action potentials • Synaptic transmission • Synaptic plasticity • Central pattern generation for rhythmic motor activity

Skills to be covered: • Neuromuscular Intro - Preparations used in Crayfish Motor Nerve, Resting Potential, Axonal conduction velocity; Rhythmic Motor Activity and Synaptic labs  • Dissecting Tips - General advice on successful dissection • Recording Tips - Background and troubleshooting for extra- and intracellular computer based data acquisition • Data Analysis - including spike sorting, matching presynaptic action potentials with postsynaptic potentials; measuring AP conduction velocity; calculating time constants for sensory receptor adaptation

FRUITFLY: These exercises will introduce educators to modern neurogenetic techniques in the fruit fly Drosophila melanogaster.

Topics to be covered:• Drosophila genetics• Optogenetics• ERG recording• Animal behavior• Synaptic transmission • Synaptic plasticity

Skills to be covered: • Generating and rearing transgenic Drosophila• Using tissue specific genetic expression techniques• Building inexpensive systems for optogenetic experiments• Quantitative analysis of animal behavior• Recording excitatory junctional potentials at the larval neuromuscular junction• Recording electroretinograms from wild type and mutant animals• Recording action potential in flight muscles through optogenetic stimulation of the Giant Fiber system

**A detailed schedule will be provided one week before the workshop. For earlier schedule details contact Bruce Johnson (brj1@cornell.edu)

 

ADInstruments is pleased to announce our Neurobiology Educator Development Scholarship Award to help offset the costs of attendance and travel to any ADInstruments-hosted neurobiology workshop.

Please visit our Neurobiology Scholarship Application Webform to learn more about the award requirements and to submit your proposal. Two scholarships will be awarded. All application materials must be submitted no later than 11:59 PM (MST) November 28th, 2022. You do not need to register for the course to apply for this scholarship. Seats to the CrawFly course are being held specifically for recipients of the scholarship. Scholarship winners will be announced by December 5th, 2022.

 

Schedule

E.g., 1 Dec 2022
E.g., 1 Dec 2022

CrawFly Workshop January 2023

4th January - 8th January 2023, 8:30am to 5:00pm

Pomona College