CrawFly Invertebrate Neurophysiology Course

Introduction


ADInstruments and the Department of Neurobiology and Behavior at Cornell University will co-host a 5 day hands-on workshop for higher education life science instructors seeking to expand their curriculum reach in the neurosciences.

The training event will be based on topics explored in the “Crawdad Project,” a three-year program funded by the National Science Foundation to promote the use of invertebrates in undergraduate physiology and neuroscience lab courses. The goals of this hosted workshop are to provide teachers hands-on experience with invertebrate preparations that demonstrate basic principles of nervous system physiology, and can easily be incorporated into laboratory courses back home.

Schedule

E.g., 21 Nov 2017
E.g., 21 Nov 2017

June 2018

20 Jun 18 to 24 Jun 18, 8:00am to 5:00pm

Comstock Hall, Ithaca, NY 14853 United States

Details


CRAWDAD (June 21-23)

Topics to be covered:
• Motor innervation 
• Neuroanatomy 
• Sensory systems 
• Ionic bases of resting and action potentials 
• Synaptic transmission 
• Synaptic plasticity 
• Central pattern generation for rhythmic motor activity

Skills to be covered: 
• Neuromuscular Intro - Preparations used in Crayfish Motor Nerve, Resting Potential, Axonal conduction velocity; Rhythmic Motor Activity and Synaptic labs  
• Dissecting Tips - General advice on successful dissection 
• Recording Tips - Background and troubleshooting for extra- and intracellular computer based data acquisition 
• Data Analysis - including spike sorting, matching presynaptic action potentials with postsynaptic potentials; measuring AP conduction velocity; calculating time constants for sensory receptor adaptation

FRUITFLY (June 23-25)
These exercises will introduce educators to modern neurogenetic techniques in the fruit fly Drosophila melanogaster.

Topics to be covered:
• Drosophila genetics
• Optogenetics
• ERG recording
• Animal behavior
• Synaptic transmission 
• Synaptic plasticity

Skills to be covered: 
• Generating and rearing transgenic Drosophila
• Using tissue specific genetic expression techniques
• Building inexpensive systems for optogenetic and thermogenetic experiments
• Quantitative analysis of animal behavior
• Recording excitatory junctional potentials at the larval neuromuscular junction
• Recording electroretinograms from wild type and mutant animals
• Recording action potential in flight muscles through optogenetic stimulation of the Giant Fiber system