When ordering, please specify the following: Mains Power (V) = 115 for 110-115 V or 220 for 220-240 V
The Dual Channel Epithelial Voltage Clamp (50 V compliance) is a state-of-the-art instrument with several unique and important design features offering more reliable recording and operator convenience.
Differential voltage recordings are made with very high common mode rejection providing accurate measurements free from the effects of common mode potential changes of a noisy environment.
Membrane Resistance Measurement:
Accurate resistance measurements are made with the membrane mounted in the chamber. This measurement is made using a low frequency 2 Hz bipolar signal to avoid polarization of the membrane (ideal for monolayers). Resistances up to 200 kΩ are displayed on the meter with push-button convenience.
Clamp Speed Selection:
Three clamp speeds provide optimum recording conditions for a variety of applications. In Fast mode, preparations with low access resistance(small tissues or monolayers) can be clamped with speeds as fast as 10µsec. Typical Ussing chambers with larger tissues will use Medium or Slow modes for stable, oscillation free clamping.
Internal DC Command (Hold) control for both Voltage clamp and Current clamp modes.
Watertight Headstage with Model Membrane:
The small compact headstage can be located close to the measurement site to keep input leads short for reduced noise pick-up. The model membrane circuit simulates a preparation to provide convenient operational checks of the clamp. Internal circuits are protected against the invasion of corrosive saline solutions by a watertight seal.
Choice of Voltage Compliance:
The high voltage compliance of this instrumentis important for studies of low resistance (leaky) epithelial cells and in applications in which long agar leads in the current passing circuit produce large voltage drops (which must be compensated). Additionally, the high compliance helps in charging large membrane capacitances typical of epithelial tissues, resulting in faster settling times and improved overall clamp performance.
The clamp can be operated by an external programmer, lab timer or computer. Logic control of clamp mode and clamp command levels is possible, as well as simultaneous mixing of external linear commands.
Onboard Timer Controller:
The Dual Channel Voltage Clamp includes event timers (2) to provide cycle times and clamp durations up to 2000 seconds. Times are set with 2 digit thumb wheel switches and 4 position range switches. Once set, the timer will free run, eliminating the need for a computer or other external device to control the experiment.