Intracellular Electrometer

Get a quote

Thank you for your interest in Intracellular Electrometer. Please complete and submit the form below and an ADInstruments representative will aim to get in touch within one working day. If you’d like to speak to someone immediately, office contact information is available from the contact page.

×

Ask a question

Thank you for your interest in Intracellular Electrometer. Please complete and submit the form below and an ADInstruments representative will aim to get in touch within one working day. If you’d like to speak to someone immediately, office contact information is available from the contact page.

×

Request a demo

Thank you for your interest in Intracellular Electrometer. Please complete and submit the form below and an ADInstruments representative will aim to get in touch within one working day. If you’d like to speak to someone immediately, office contact information is available from the contact page.

×
×
checkmark Item successfully added to your quote request.

Intracellular Electrometer

IE-210-V

Intracellular Electrometer

PRICE ON REQUEST

Product code: IE-210-V

An intracellular microelectrode amplifier for recording from high impedance, fluid-filled glass electrodes.

Book a Demo

Overview

The Intracellular Electrometer is an intracellular microelectrode amplifier with low noise, low drift and fast response time for recording from high impedance, fluid-filled glass electrodes. Bridge circuitry permits simultaneous stimulation and recording through a single electrode. These features, in combination with a small, lightweight headstage, 4-pole Bessel low pass filter and optional probe breakaway, make it an ideal instrument for easy, accurate and reliable intracellular recording and current injection. The instrument consists of two sections: the headstage and the amplifier, which contains the signal conditioning, monitoring and power circuitry.

Included with the Intracellular Electrometer:

The Intracellular Electrometer can also be purchased as part of the Intracellular Recording System.

 Accessories purchased separately:

Details

Features


  • High impedance microelectrode amplifier for intracellular studies.
  • Simultaneous current injection stimulation and recording.
  • Bridge Balance Output gains of x10, x20, and x50.
  • Capacity Compensation for up to 50 pF.

 

The Headstage:
Fluid-filled microelectrodes can have resistances of over 100 MΩ. To faithfully record potentials with such high resistances, the amplifier input resistance must be at least 100 times greater. The input resistance of the Intracellular Electrometer is 5000 times greater (5 x 1011 Ω) to assure accurate measurements with high resistance electrodes.

To maximize the bandwidth of voltage recording with high resistance electrodes, care must be taken to limit the input capacitance of the amplifier, and to minimize stray capacitance associated with the electrode and cable connected to the amplifier. The Intracellular Electrometer​ headstage is designed to minimize these capacitance noise sources. The headstage utilizes bootstrap circuitry and a driven shield to limit input capacitance to 0.5 pF.

Stray input capacitance is reduced using two design features of the headstage:

  • The pencil-sized headstage mounts directly on your micromanipulator, allowing a direct connection to the headstage input.
  • The driven headstage shield is available at the probe input to permit attachment of additional shielding.

This approach allows the shield to be extended to enclose the electrode holder and further reduce stray input capacitances. These methods of capacitance neutralization are preferable to the more common method of capacitance neutralization since capacitance compensation circuitry can increase noise levels.

Electrometer Features:
The probe/electrometer output is available at a gain of one. Amplifier gain is increased to x10 at the current injection bridge output. Input junction potentials can be compensated with the input offset control. If desired, remaining stray input capacitance, up to 50 pF, can be neutralized using capacity compensation circuitry.

Convenient electrode resistance measurement is available using Electrode Test which produces a voltage proportional to the electrode resistance. Cell impalement is facilitated with a Buzz voltage (variable in both frequency and amplitude) applied to the electrode. An overload condition at the input (blocked electrode or oscillations caused by excessive capacity compensation) is indicated by the Input Overload light.

Current Injection:
Simultaneous stimulation and recording with a single electrode is made possible with an active bridge circuit. Stimulus currents are generated either internally with the DC Current source or by a voltage applied to the external Stimulus Input. The internal source supplies currents up to ±100 nA in the Low range and up to ±1000 nA in the High range. The current injection feature is often used for iontophoretic injection of dyes or drugs. External signals can be of any waveform or DC level. Internal and external stimulus currents are summed when applied simultaneously.

Voltage drops across the electrode are subtracted from the Bridge Output using the DC Balance and AC Balance controls. The remaining output represents the cell's response to the applied current. Electrode resistance can be read directly from the DC Balance dial. Gain of the Bridge Output signal is x10 at the front panel and can be switch-selected to x20 or x50 at the rear panel. The output signal is available at either the full amplifier bandwidth (30 kHz) or filtered with the internal 4-pole Bessel low pass filter.

Additional Features:

  • The 3-1/2 digit LED meter provides readouts of the x1. Amplifier Output voltage and the stimulus DC Current, and is easily viewed in a darkened room.
  • Checks of amplifier operation are quick and convenient with the front panel Probe Test facility, a real time-saver when troubleshooting set-up problems.
  • Signals from another amplifier can be applied to the Differential Input and are then summed with the Intracellular Electrometer​ x1 output.
  • The filter frequency is encoded by a stepped DC voltage at the Filter Telegraph BNC for input to a data acquisition system.

Product Code


IE-210-V
Intracellular Electrometer

Technical


Ordering Information 

When ordering please specify the following: Mains Power (V) = 115 for 110-115V or 220 for 220-240V

 

Specifications

  • Input Impedance: 5 x 1011 Ω shunted by 0.5 pF
  • Output Resistance: 100 Ω
  • Gain: x1 ± 0.1%
  • Probe Input Range: ±10 V
  • Risetime (10-90%): 25 µsec, measured through 20 MΩ
  • Noise Level: 25 µV p-p input shorted (0.1 Hz to 10 kHz) at x1 output & 250 µV p-p, 20 MΩ at input
  • Capacitance Compensation: 0-50 pF
  • Leakage Current: 1 pA typical, adjustable to zero
  • Input Offset: ± 200 mV, referred to input
  • Electrode Resistance Test: 1 mV/MΩ (Low Range) & 10 mV/MΩ (High Range)
  • Buzz: Amplitude variable 0 to 15 V
  • Frequency variable 100 Hz to 10 kHz

Current Injection Bridge:

Bridge operates in Low and High current ranges.

  • Bridge Resistance Ranges: 0 to 100 MΩ & 0 to 1000 MΩ (Low Range) & 0 to 10 MΩ & 0 to 100 MΩ (High Range)
  • Internal DC Current Source: 0 to ± 100 nA (Low Range) & 0 to ± 1000 nA (High Range)
  • External Stimulus: Current is limited by electrode resistance up to maximums: ± 10 V ÷ electrode R or 0.5 µA (Low Range) & ± 10 V ÷ electrode R or 5 µA (High Range)
  • Max. Stimulus Input: ± 15 V
  • Stimulus Input Resistance: 10 KΩ
  • I Monitor Output: 100 mV/nA (Low Range) & 10 mV/nA (High Range)
  • Bridge Output Gain: x10 at front panel, x20 or x50 at rear panel
  • Bridge Output Filter: 4-pole low pass Bessel with cut-offs at 0.05, 0.1, 0.2, 0.5, 1, 2, 5, 10 and 20 kHz
  • Bridge Output Resistance: 100 Ω

Additional Features:

  • Meter: 3-1/2 digit LED
  • Amplifier Output: 200 mV, 2 V and 20 V
  • Current: 200 nA and 2 µA
  • Power Requirements: 100 to 130 or 220 to 240 VAC, 50/60 Hz, 10 VA
  • Physical Dimensions -: Probe: 12.5 mm D x 6.2 cm with 1.8 m cable
  • Physical Dimensions - Case: 8.9 x 43.2 x 30.5 cm (H x W x D)
  • Shipping Weight: 5.5 kg
  • Warranty: Three years, parts and labor