The Langendorff Apparatus & Thermostat Controller is a compact, self-contained and thermostatically-controlled unit, which is ideal for the retrograde perfusion (Langendorff) of isolated small rodent (mice and rats) hearts. Its design allows the full immersion of two separate perfusate reservoirs, respective coiled tubes heat exchangers in a compact water bath that is heated to and kept at constant temperature. The water bath has an automatic water level detection safety switch that stops heating when insufficient water, and a lid for minimizing evaporation and heat loss. Perfusate temperature is further maintained via a heated junction block before it reaches the heart cannula.
Having the option of two separate perfusate reservoirs with a 3-way valve switch allows
- both perfusates to be similarly buffered at the same pH and maintained at the same temperature.
- quick and easy switch between the two reservoirs.
- an instant available secondary primed perfusate preventing any fluctuation in the perfusate condition when primary reservoir is almost empty or being refilled.
- prevents the risk of introducing air bubbles into the circulation upon reservoir change.
- an option to have two interchangeable perfusates with different compositions.
With Minipuls 3 Peristaltic Pump, STH Pump Controller and compatible accessories (all purchased separately), the perfusate can be pressurized to a target pressure and pumped through the coiled tubes heat exchangers, heated junction block, and then into an attached cannulated isolated heart. Physiological parameters of the heart can then be measured and recorded via suitable sensors, transducers, and a PowerLab data acquisition system (all purchased separately).
This unit is also available as part of the compact Langendorff System – Panlab (Constant Pressure or Flow). Alternatively, other Langendorff apparatuses (constant perfusion pressure or flow) and systems are also available. For example, it is highly recommended for other Langendorff perfusion systems to be used with larger isolated hearts, such as rabbits or cats.