NaviCyte Vertical Ussing Chamber System
The NaviCyte Vertical Ussing Chamber System is designed to simultaneously maintain and run up to six Ussing chambers in a single holder. The holder is paired with suitable epithelial voltage clamp systems to measure epithelial electrophysiological parameters.
Overview
The NaviCyte Vertical Ussing Chamber System is designed to simultaneously maintain and run up to six NaviCyte Vertical Ussing Chambers in a single holder. Pairing with suitable epithelial voltage clamp systems allows electrophysiological and diffusion-only measurements on both grown monolayers (Snapwell cell cultures) and surgically excised tissue sections, for quantification and analysis of transport and barrier functions.
A fully comprehensive system can be easily and individually tailored by selecting:
- PowerLab 8/35 or 16/35 and LabChart Pro software
- 19 Inch Rack Adapter
- the required number of Single or Dual Epithelial Voltage Clamp Amplifiers
- NaviCyte Heating Block Assembly (Vertical, 6 Chamber) if 2-6 simultaneous recordings are required
- the required type and number of NaviCyte Vertical Ussing Chambers
- the corresponding type and number of chamber caps
- any required electrodes and accessories
- a basic external thermal water recirculator (connectors are required and vary depending on the diameter of the recirculator inflow and outflow)
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Key Features
The system centers around the NaviCyte Heating Block Assembly (Vertical, 6 Chamber), which functions as:
- a visually unobtrusive holder of up to six NaviCyte Vertical Ussing Chambers
- a compact holder (38.1 x 10.2 x 12.7 cm) that conserves valuable bench space
- a heater block base that provides efficient temperature regulation of the chambers from an external thermal water circulator (purchased separately)
- a gas manifold that distributes gas to both sides of each chamber, with individual side chamber delivery control
Additional features include:
- employing reusable electrode glass barrels with ceramic frits that save cost
- a variety of different chambers types (up to 13)
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Details and Tech Specs
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Requirements
How to configure your NaviCyte Vertical Ussing System:
- Select NaviCyte Heating Block Assembly (Vertical, 6 Chamber) if more than one chamber or if heating is required
- Select required NaviCyte Vertical Ussing Chambers – up to 6
- Select corresponding chamber caps (one set per chamber)
- Select Perfusion Caps – if perfusion is required in standard volume chambers (not required if recording electrophysiological measurements, or using Electrode Caps, or using low volume chambers)
- Select Electrode Caps – if recording electrophysiological measurements in standard volume chambers
- Select Snapwell Electrode Caps – if using Snapwell chambers and recording electrophysiological measurements
- Select NaviCyte Ussing Chamber Ag/AgCl Electrode Set (one set per chamber) and NaviCyte Electrode Glass Barrels (8pk) - if recording electrophysiological measurements
- Select appropriate Heated Water Circulator, tubing, and tubing accessories – if not available in the laboratory and temperature maintenance is required
- Select appropriate amplifiers, interface cables, and PowerLab and LabChart data acquisition system
