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Oocyte Clamp Amplifier
OC-725D-V
Oocyte Clamp Amplifier
PRICE ON REQUEST
Product code: OC-725D-V
Designed for two-electrode voltage clamping (TEVC) of Xenopus oocytes and other large cells.
Overview
An amplifier designed for two-electrode whole-cell voltage clamping of large cells (Xenopus oocytes) and cell structures (squid axons). Measurements can then be recorded via a PowerLab and LabChart data acqusition system, which is purchased separately. This two-electrode voltage clamping (TEVC) amplifier features:
- High AC and DC gains
- High compliance voltage (±180 V) - ensuring fast, non-saturating clamp performance under nearly any condition
- Two clamp speeds (Slow or Fast)
- Improved Bath Clamp Headstage – extended current measuring range
- amplifies smaller currents to usable levels
- records larger currents (up to 1 mA) without output saturation
- Unique bath clamp circuitry - eliminates the need for series resistance compensation, and accurately measures bath current
- Simultaneous displays of membrane voltage (Vm) and membrane current (Im)
- Buzz controls (1 kHz square wave) - aids in penetration of cell membranes with a minimum of leakage
- Capacitive compensation for the Vm
Supplied with:
- Voltage Headstage
- Bath Clamp Headstage
- Current Electrode Cable
- Oocyte Model Cell
- Rack mount accessories
Accessories (purchased separately):
- Oocyte Recording Chamber
- Clamp Mount Micromanipulators (Left and Right)
- Magnetic Base for Clamp Micromanipulator (x2)
- Magnetic Steel Base Plate (305x610x9.5mm)
- E Series Electrode Holders
The Oocyte Clamp Amplifier is supplied when purchasing:
Features
- High compliance voltage (±180 V)
- Unique bath clamp circuitry
- Ideal for clamping large cells and cell structures, e.g. squid axons
- Extended current measuring range
- Decreased noise level and 4-pole Bessel filter
- An internal switch permits measurements of the current in series with the current electrode, instead of in the bath
- Optional differential voltage headstages
- Buzz controls (1 kHz square wave) for each electrode aid in penetration of cell membranes with a minimum of leakage
- Overload alarm (audible and visual) indicate when the compliance voltage is exceeded safeguarding the oocyte and indicating that current records are subject to saturation
- DC Offsets for both voltage and current electrodes
- Electrode Test for both electrodes
- Capacity Compensation for the Vm voltage input
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Technical
Ordering Information
When ordering the Oocyte Clamp Amplifier please specify the following: Mains Power (V) = 115 for 110-115 V or 220 for 220-240 V.
Oocyte Clamp Amplifier Specifications
| Test Condition #1 | Model cell used to obtain specifications.
Model membrane: 1 MΩ in parallel with 220 nF. Current and voltage electrodes both 1 MΩ impedance. |
|
|---|---|---|
| Test Condition #2 | Noise measurements made with an 8-pole Bessel filter. | |
| Voltage Recording Channel (Vm) | V Probe Input Impedance | 0.5 x 1012Ω, 1 pF |
| Output Resistance | 100Ω | |
| DC Offset | ± 200 mV at input. Variations from zero with 10 turn control (20 mV/turn). | |
| Noise (0-10 kHz) | 3 µV RMS with input grounded 20 µV RMS with model cell |
|
| Electrode Test | 10 mV/MΩ read on meter 100 mV/MΩ at Vm x10 output |
|
| Meter Range | ± 199.9 mV full scale | |
| Capacity Compensation | 0 - 90 pF | |
| Current Sensing Channel (Bath Clamp) (Im) | Noise (0-1 kHz) | 4.5 nA RMS with bath clamp
10 nA RMS in output leg |
| I Monitor Output | 1 nA/mV to 1 mA/10V in 7 steps and 3 ranges, x0.1, x1, and x10. | |
| Gain Telegraph Output | 0.2 to 2.8 V in 8 steps (200 mV/step) and 3 ranges, x0.1, x1, and x10. Compatible with data acquisition software |
|
| Meter Range, full scale | Clamp Current: ± 199.9 µA | |
| Electrode Voltage (Ve) | ± 199.9 mV; current meter reads Ve when clamp mode switch is off. | |
| Current Electrode Channel | Compliance Voltage | ± 180 V |
| Clamp Speed | 550 µsec; (10-90%) with 100 mV square wave command applied to model cell | |
| Gain | Variable AC/DC: 0 to 2000 | |
| Fixed DC Gain | Switch selected: 1 x 106 | |
| Ve DC Offset | ± 200 mV at input (20 mV/turn) | |
| Electrode Test | 10 mV/MΩ read on current meter
100 mV/MΩ at Ve x10 output rear panel |
|
| Commands | Hold | Manually set with digital potentiometer, 2 digit resolution and 2 ranges: x10 range: 0 to ± 100 mV x20 range: 0 to ± 200 mV |
| External | Signals applied to COMMAND IN÷10 are attenuated by a factor of 10, 1 V applied = 100 mV command |
|
| Physical Dimensions | Case | 8.9 cm H x 43.2 cm W x 30.5 cm D |
| Voltage Headstage | 12.5 mm D x 5 cm L with 1.8 m cable | |
| Mounting Handle | 4.8 mm D x 6.3 cm L | |
| Bath Headstage | 2.3 cm H x 3.5 cm W x 4.2 cm L with 1.8 m cable | |
| Shipping Weight | 6.0 kg | |
| Warranty | One year, parts & labor | |
| Power Requirements | Universal: 100-130 or 220-240 VAC, 50/60 Hz, 14 VA | |
