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Oocyte Clamp Amplifier

OC-725C-V

Oocyte Clamp Amplifier

PRICE ON REQUEST

Product code: OC-725C-V

Designed for two-electrode voltage clamping (TEVC) of Xenopus oocytes and other large cells.

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Overview

An amplifier designed for two-electrode whole-cell voltage clamping of large cells (Xenopus oocytes) and cell structures (squid axons). Measurements can then be recorded via a PowerLab and LabChart data acqusition system, which is purchased separately. This two-electrode voltage clamping (TEVC) amplifier features:

  • High AC and DC gains
  • High compliance voltage (±180 V) - ensuring fast, non-saturating clamp performance under nearly any condition
  • Two clamp speeds (Slow or Fast)
  • Improved Bath Clamp Headstage – extended current measuring range
    • amplifies smaller currents to usable levels
    • records larger currents (up to 1 mA) without output saturation
  • Unique bath clamp circuitry - eliminates the need for series resistance compensation, and accurately measures bath current
  • Simultaneous displays of membrane voltage (Vm) and membrane current (Im)
  • Buzz controls (1 kHz square wave) - aids in penetration of cell membranes with a minimum of leakage
  • Capacitive compensation for the Vm
     

Supplied with:

Accessories (purchased separately):

The Oocyte Clamp Amplifier is supplied when purchasing:

Features

  • High compliance voltage (±180 V)
  • Unique bath clamp circuitry
  • Ideal for clamping large cells and cell structures, e.g. squid axons
  • Extended current measuring range
  • Decreased noise level and 4-pole Bessel filter
  • An internal switch permits measurements of the current in series with the current electrode, instead of in the bath
  • Optional differential voltage headstages
  • Buzz controls (1 kHz square wave) for each electrode aid in penetration of cell membranes with a minimum of leakage
  • Overload alarm (audible and visual) indicate when the compliance voltage is exceeded safeguarding the oocyte and indicating that current records are subject to saturation
  • DC Offsets for both voltage and current electrodes
  • Electrode Test for both electrodes
  • Capacity Compensation for the Vm voltage input

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Details and Tech Specs

Technical

Ordering Information

When ordering the Oocyte Clamp Amplifier please specify the following: Mains Power (V) = 115 for 110-115 V or 220 for 220-240 V.

Oocyte Clamp Amplifier Specifications

Test Condition #1 Model cell used to obtain specifications.
Model membrane: 1 MΩ in parallel with 220 nF.
Current and voltage electrodes both 1 MΩ impedance.
Test Condition #2 Noise measurements made with an 8-pole Bessel filter.
Voltage Recording Channel (Vm) V Probe Input Impedance 0.5 x 1012Ω, 1 pF
Output Resistance 100Ω
DC Offset ± 200 mV at input. Variations from zero with 10 turn control (20 mV/turn).
Noise (0-10 kHz) 3 µV RMS with input grounded
20 µV RMS with model cell
Electrode Test 10 mV/MΩ read on meter
100 mV/MΩ at Vm x10 output
Meter Range ± 199.9 mV full scale
Capacity Compensation 0 - 90 pF
Current Sensing Channel (Bath Clamp) (Im) Noise (0-1 kHz) 4.5 nA RMS with bath clamp
10 nA RMS in output leg
I Monitor Output 1 nA/mV to 1 mA/10V in 7 steps and 3 ranges, x0.1, x1, and x10.
Gain Telegraph Output 0.2 to 2.6 V in 7 steps (200 mV/step) and 3 ranges, x0.1, x1, and x10. Compatible with data acquisition software*
Meter Range, full scale Clamp Current: ± 199.9 µA
Electrode Voltage (Ve) ± 199.9 mV; current meter reads Ve when clamp mode switch is off.
Current Electrode Channel Compliance Voltage ± 180 V
Clamp Speed 350 µsec; (10-90%) with 100 mV square wave command applied to model cell
Gain Variable AC/DC: 0 to 2000
Fixed DC Gain Switch selected: 1 x 106
Ve DC Offset ± 200 mV at input (20 mV/turn)
Electrode Test 10 mV/MΩ read on current meter
100 mV/MΩ at Ve x10 output rear panel
Commands Hold Manually set with digital potentiometer, 2 digit resolution and 2 ranges:
x1 range: ± 1 to 99 mV in 1 mV steps
x2 range: ± 2 to 198 mV in 2 mV steps
External Signals applied to COMMAND IN÷10 are attenuated by a factor of 10,
1 V applied = 100 mV command
Physical Dimensions Case 8.9 cm H x 43.2 cm W x 30.5 cm D
Voltage Headstage 12.5 mm D x 5 cm L with 1.8 m cable
Mounting Handle 4.8 mm D x 6.3 cm L
Bath Headstage 2.3 cm H x 3.5 cm W x 4.2 cm L with 1.8 m cable
Shipping Weight 6.8 kg
Warranty Three years, parts & labor
Power Requirements 100-130 V or 220-240 VAC, 50/60 Hz, 20 VA

Product Code

OC-725C-V
Oocyte Clamp Amplifier
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