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Two Electrode Voltage Clamp Workstation

TEV700-V

Two Electrode Voltage Clamp Workstation

PRICE ON REQUEST

Product code: TEV700-V

Provides two-electrode voltage clamping via an Oocyte Clamp Amplifier and the necessary components typically used with in research involving Oocytes.

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Overview

This workstation provides two-electrode voltage clamping (TEVC) via an Oocyte Clamp Amplifier and many of the necessary components typically used with in electrophysiology research involving Oocytes. Measurements can then be recorded via a PowerLab and LabChart data acqusition system, which is purchased separately.

Included with the workstation are: 

Two E series electrode holders are typically required with the clamp and varying sizes can be  purchased separately:

  • (Str, Vent, Ag Wire) for use with the voltage headstage 
  • (45°, Vent, Handle, Ag Wire) for use with the current electrode 


This workstation can also be purchased as part of the Two Electrode Voltage Clamp Workstation System.

Accessories (optional, purchased separately):

  • Magnetic Steel Base Plate (305x610x9.5mm) 

Related

Research Applications

Features

  • High compliance voltage (±180 V)
  • Unique bath clamp circuitry
  • Ideal for clamping large cells and cell structures, e.g. squid axons
  • Extended current measuring range
  • Decreased noise level and 4-pole Bessel filter
  • An internal switch permits measurements of the current in series with the current electrode, instead of in the bath
  • Optional differential voltage headstages
  • Buzz controls (1 kHz square wave) for each electrode aid in penetration of cell membranes with a minimum of leakage
  • Overload alarm (audible and visual) indicate when the compliance voltage is exceeded safeguarding the Oocyte and indicating that current records are subject to saturation
  • DC Offsets for both voltage and current electrodes
  • Electrode Test for both electrodes
  • Capacity Compensation for the Vm voltage input

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Details and Tech Specs

Technical

Oocyte Clamp Amplifier Specifications

Test Condition #1 Model cell used to obtain specifications. Model membrane: 1 MΩ in parallel with 220 nF. Current and voltage electrodes both 1 MΩ impedence.
Test Condition #2 Noise measurements made with an 8-pole Bessel filter.
Voltage Recording Channel (Vm) V Probe Input Impedance 0.5 x 1012 &, 1 pF
Output Resistance 100 Ω
DC Offset ± 200 mV at input. Variations from zero with 10 turn control (20 mV/turn).
Noise (0-10 kHz) 3 µV RMS with input grounded. 20 µV RMS with model cell.
Electrode Test 10 mV/MΩ read on meter 100 mV/MΩ at Vm x10 output.
Meter Range ± 199.9 mV full scale
Capacity Compen 0 - 90 pF
Current Sensing Channel (Bath Clamp) (Im)

Noise (0-1 kHz) 4.5 nA RMS with bath clamp
10 nA RMS in output leg
I Monitor Output 1 nA/mV to 1 mA/10V in 7 steps and 3 ranges, x0.1, x1, and x10.
Gain Telegraph Output 0.2 to 2.6 V in 7 steps (200 mV/step) and 3 ranges, x0.1, x1, and x10. Compatible with data acquisition software*
Meter Range, full scale Clamp Current: ± 199.9 µA
Electrode Voltage Ve ± 199.9 mV; current meter reads Ve when clamp mode switch is off.
Current Electrode Channel Compliance Voltage ± 180 V
Clamp Speed 350 µsec; (10-90%) with 100 mV square wave command applied to model cell.
Gain Variable AC/DC: 0 to 2000
Fixed DC Gain Switch selected: 1 x 106
Ve DC Offset ± 200 mV at input (20 mV/turn)
Electrode Test 10 mV/MΩ read on current meter.
100 mV/MΩ at Ve x10 output rear panel
Commands Hold Manually set with digital potentiometer, 2 digit resolution and 2 ranges:
x1 range: ± 1 to 99 mV in 1 mV steps
x2 range: ± 2 to 198 mV in 2 mV steps
External Signals applied to COMMAND IN÷10 1 V applied = 100 mV
Physical Dimensions Case 8.9 cmx 43.2 cmx 30.5 cm
Voltage Headstage 12.5 mm D x 5 cm L with 1.8 m cable
Mounting Handle 4.8 mm D x 6.3 cm L
Bath Headstage 2.3 cm x 3.5 cm x 4.2 cm with 1.8 m cable
Shipping Weight 6.8 kg
Warranty Three years, parts & labor
Power Requirements 100-130 V or 220-240 VAC, 50/60 Hz, 20 VA

Oocyte Recording Chamber Specifications

Material Polycarbonate
Footprint, L x W 13 x 7.7 cm (5.1 x 3 in)
Bath Dimensions
Narrow Bath 27 x 3.2 x 3.2 mm
Volume ~85 µl/mm Height
Wide Bath 27 x 6.3 x 3.2 mm
Volume ~170 µl/mm Height
Chamber Floor Insert 25 x 75 x 1 mm Polycarbonate
Input Tubing Size 1/16 in OD (PE-160)
Output Tubing Size 1/16 in ID

Product Code

TEV700-V
Two Electrode Voltage Clamp Workstation

Related

Research Applications

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