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Intracellular

Intracellular

Intracellular recording is an electrophysiology technique that inserts a microelectrode into a single cell (usually a neuron) to precisely measure its electrical activity - apparent as voltages across, or currents passing through, the cellular membranes. It can also be used to measure slower graded voltages, like receptor potentials or synaptic potentials, which are vital in neural signaling studies.

Intracellular techniques use Ohm’s Law to focus on certain aspects of electrical activity and can be differentiated by the type of glass microelectrode used. This could either be a sharp electrode (with a relatively small, fine tip) or a blunt, or patch, electrode (with a large tip).

Follow the cell membrane potential at rest and in firing cells by using PowerLab to record slow events (e.g., miniature postsynaptic potentials) as well as fast events (e.g., action potentials), or sample membrane currents in voltage clamp mode. Our intracellular microelectrode amplifiers provide the instrumentation you require.

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Intracellular action potentials analyzed with Peak Analysis

Products and Solutions

Record multiple,<br />
high-quality signals<br />
at one time

Record multiple,
high-quality signals
at one time

Powerful &<br />
comprehensive<br />
systems

Powerful &
comprehensive
systems

Integrate your<br />
data streams<br />
in one place

Integrate your
data streams
in one place

Mix & match<br />
products for a<br />
custom solution

Mix & match
products for a
custom solution

In conjunction with our solutions shown below, any device with an analog output (+/- 10V) can be connected to a PowerLab data acquisition system for synchronization of the event in LabChart, giving you even more flexibility and the ability to integrate your data streams in one place.

All our hardware is backed by the appropriate safety certification standards. More information on safety standards.

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Components & Related Products

Typical Intracellular Studies

Neurophysiology

Intracellular homeostasis

Ion channel receptors and ion transport

Neuronal mechanisms

Signals Supported

Intracellular Recordings
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A range of voltage and current signals can be recorded from neurons, which can be grouped as Intracellular Recordings. These include signals from sharp electrodes like Membrane Potential/Voltage (Current or Voltage Clamping) and Membrane Current (Voltage Clamping). They also include signals generated from Patch Clamping.

Products related to Intracellular Recordings and Intracellular

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Flexible Data Acquisition

ADInstruments systems provide an integrated solution to advance life science research. With the combination of LabChart analysis software and a PowerLab data acquisition unit you have the flexibility to collect and synchronize a wide range of signals for analysis. We also offer a range of LabChart compatible solutions able to stream directly in LabChart.

LabChart

LabChart

Enabling Discovery

LabChart data analysis software creates a platform for all of your recording devices to work together, allowing you to acquire biological signals from multiple sources simultaneously and apply advanced calculations and plots as your experiment unfolds.

LabChart and Intracellular

PowerLab

LabChart

Data with Integrity

High-performance data acquisition hardware designed for life science research. PowerLab is engineered for precise, consistent, reliable data acquisition, giving you the reproducible data you need while meeting the strictest international safety standards.

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All your Intracellular analysis in one place

LabChart software is designed specifically for life science data and provides up to 32 channels for data display and analysis options that are powerful and easy to use. With auto-recognition of ADI and LabChart Compatible hardware, multi-window views, one touch recording, simultaneous recording from multiple devices, specialized preconfigured settings, easy sharing options and an interface that can be customized to show only the features you want to use.

  • Intracellular action potentials analyzed with Peak Analysis

    Intracellular action potentials analyzed with Peak Analysis

Noise Elimination

This is a common issue in electrophysiology as you are dealing with very small signals as well as high impedance and capacitance of electrodes. All of ADInstruments hardware is optimized with high-quality noise specifications, but it is always good to have a few other tricks up your sleeve to help in your setup. 

  1. Ensure all electronic equipment used in recording setup is plugged into the same wall socket by using a fused multibox.
  2. Ensure all shielding is connected to one grounding point or grounding bar. This will eliminate the risk of a grounding loop when different grounds are at slightly different potentials causing currents to flow in the shielding introducing noise.
  3. To identify the source of noise, strip back to the bare essentials, like the amplifier and acquisition and then systematically switch on additional equipment.
  4. Always try to eliminate the source of noise first, but if this isn’t possible then mask with shielding.
  5. Try and keep lead wires as short as possible and not looped. If they are long, try braiding them to prevent magnetically induced currents.
  6. Even fluid filled tubing for perfusions can act as antennas. If this is the case, shield with tin foil and connect to the metal grounding bar.
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Features and Add-Ons

Additional acquisition and analysis features and add-ons to support your Intracellular studies:

Explore Intracellular Data in LabChart Reader

LabChart Reader is a free application for conducting limited analyses of LabChart data. Download a trial package with example data below.

Platform Required

Thanks! Your trial package is now downloading.

Once downloaded, please unzip the folder and install the LabChart Reader application.

Open the "LabChart Data Files" folder to find data files (".adicht"). Data files can be opened in LabChart Reader by double clicking on the file name.

Request a demo

Thank you for your interest in Intracellular. Please complete and submit the form below and an ADInstruments representative will aim to get in touch within one working day. If you’d like to speak to someone immediately, office contact information is available from the contact page.

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Ask a question

Thank you for your interest in Intracellular. Please complete and submit the form below and an ADInstruments representative will aim to get in touch within one working day. If you’d like to speak to someone immediately, office contact information is available from the contact page.

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