×

Ussing

Ussing

The Ussing chamber, which was invented by Hans Henriksen Ussing, is used for housing and sustaining living epithelial tissue or a monolayer of cells, while an appropriate epithelial amplifier and suitable electrodes are used to measure the electrical properties of the membrane. These electrical properties indicate and quantify the transport of ions, nutrients, and drugs across the tissue.

Ask a Question Book a Demo

Recorded short-circuit current (Isc) from two epithelial tissues maintained Ussing chambers in response to a drug agonist and are superimposed via Scope View.

Products and Solutions

Record multiple<br />
signals at one time

Record multiple
signals at one time

Simple, streamlined systems<br />
you can build upon

Simple, streamlined systems
you can build upon

Mix and match products for a custom solution

Mix and match products for a custom solution

Integrate all your<br />
data streams<br />
in one place

Integrate all your
data streams
in one place

In conjunction with our solutions shown below, any device with an analog output (+/- 10V) can be connected to a PowerLab data acquisition system for synchronization of the event in LabChart, giving you even more flexibility and the ability to integrate your data streams in one place.

All our hardware is backed by the appropriate safety certification standards. More information on safety standards.

Complete System

Click the Quote Request icon to add
a product to your Quote Request

×

Components & Related Products

Typical Ussing Chamber Studies

Epithelial ion transport

Drug absorption

Transmucosal studies

Transmembrane conductance

Epithelial permeability

Gastrointestinal studies

Skin studies

Signals Supported

A range of signals can be recorded from living epithelial tissue or a monolayer of cells:

Extracellular Recordings
+

Perform Epithelial Voltage Clamping using our complete systems for accurate measurements of transepithelial voltage, short circuit current, and membrane resistance.

Products related to Extracellular Recordings and Ussing

×
Temperature
+

Temperature can be recorded in Ussing research.

Products related to Temperature and Ussing

×

Flexible Data Acquisition

ADInstruments systems provide an integrated solution to advance life science research. With the combination of LabChart analysis software and a PowerLab data acquisition unit you have the flexibility to collect and synchronize a wide range of signals for analysis. We also offer a range of LabChart compatible solutions able to stream directly in LabChart.

LabChart

LabChart

Enabling Discovery

LabChart data analysis software creates a platform for all of your recording devices to work together, allowing you to acquire biological signals from multiple sources simultaneously and apply advanced calculations and plots as your experiment unfolds.

LabChart and Ussing

PowerLab

LabChart

Data with Integrity

High-performance data acquisition hardware designed for life science research. PowerLab is engineered for precise, consistent, reliable data acquisition, giving you the reproducible data you need while meeting the strictest international safety standards.

Learn more

All your Ussing analysis in one place

LabChart software is designed specifically for life science data and provides up to 32 channels for data display and analysis options that are powerful and easy to use. With auto-recognition of ADI and LabChart Compatible hardware, multi-window views, one touch recording, simultaneous recording from multiple devices, specialized preconfigured settings, easy sharing options and an interface that can be customized to show only the features you want to use.

  • Recorded short-circuit current (Isc) from two epithelial tissues maintained Ussing chambers in response to a drug agonist and are superimposed via Scope View.

    Recorded short-circuit current (Isc) from two epithelial tissues maintained Ussing chambers in response to a drug agonist and are superimposed via Scope View.

Ensuring stable recordings of short-circuit currents

Here are some tips to ensure you get stable recordings of short-circuit currents:

  • Minimize solution evaporation, such as when bubbling with the 5% CO2 dry gas mixture; - which can lead to an upward drift of solution osmolarity.
  • Prevent electrolyte (KCl) leakage from the agar bridges, which will also increase osmolarity with time.
  • Ensure smooth solution flow or exchange, to minimize sudden changes to chamber solution content, which can produce artifacts.
  • Maintain consistent temperature and minimize external heat loss and temperature fluctuation by insulating exposed surfaces of the chamber or lines where feasible.
     
×

Features and Add-Ons

Additional acquisition and analysis features and add-ons to support your Ussing studies:

Explore Ussing Data in LabChart Reader

LabChart Reader is a free application for conducting limited analyses of LabChart data. Download a trial package with example data below.

Platform Required

Thanks! Your trial package is now downloading.

Once downloaded, please unzip the folder and install the LabChart Reader application.

Open the "LabChart Data Files" folder to find data files (".adicht"). Data files can be opened in LabChart Reader by double clicking on the file name.

Citations

2,410 papers

in Ussing on Google Scholar cite ADInstruments.

Request a demo

Thank you for your interest in Ussing. Please complete and submit the form below and an ADInstruments representative will aim to get in touch within one working day. If you’d like to speak to someone immediately, office contact information is available from the contact page.

×

Ask a question

Thank you for your interest in Ussing. Please complete and submit the form below and an ADInstruments representative will aim to get in touch within one working day. If you’d like to speak to someone immediately, office contact information is available from the contact page.

×