A saline bolus aims to achieve a sudden increase in measured volume while maintaining a stable pressure signal. This provides the data required by the software to eliminate the parallel volume of the myocardium. The response to the bolus should look like below:
A mixture of 15-30% hypertonic saline solution is typically injected through the jugular vein. When collecting PV data from the left ventricle, the left jugular is suggested for ease of surgery.
- Typical injection ranges for mice are 5-10 µL
- Typical injection ranges for rats are 20-40 µL
Animal size, strain bolus volume, and solution strength may vary depending on the injection site. It may be necessary to increase/decrease the amount of saline injected, the speed of injection, and the percent of salt within the saline.
For further technical assistance with this or any other issue, please contact ADInstruments Technical Support by clicking HERE.