What is the expected temperature differential between the water in the Water Bath and water in the Junction Block of the Compact Langendorff System?

The temperature difference between the water in Water Bath and water in the Junction Block is typically between 2 °C to 3 °C at the ambient room temperature of 22 °C. 

Protocol to measure the water temperature difference between water bath and junction block

1. Connect the Heart Chamber, Junction Block and Thermostat to the Langendorff Apparatus (refer to the Langendorff system owner’s guide if required). 
   
2. Fill the Water Bath with water and and then switch on the pump.
   
3. Prime the Heart Chamber – Junction Block circuit with water.
   
4. Switch ON the Thermostat and set the temperature to 37 °C.
   
5. Once the temperature of the water in the Water Bath reaches 37°C, turn off the left middle Luer connector and remove the silicon tube from left middle Luer connector (refer to the picture below).
   
6. Using a thermocouple, measure the temperature of the water coming out of the silicon tube (outlet of junction block) directly before collecting in a beaker.