How do I measure aortic pressure in a Langendorff Heart preparation?

'Perfusion pressure' and 'aortic pressure' are two terms used to describe one of the pressure parameters in a Langendorff Heart experiment.


Described below is a quick guide describing the technique used to measure the perfusion/aortic pressure using a pressure transducer.


Firstly, the perfusate in the perfusate reservoir is typically gassed with 95% O2 and 5% CO2 for at least 30 minutes. The perfusate then flows into the aorta of the heart via a 3-way tap. The catheter of the pressure transducer is also connected to the 3-way tap, which allows constant measurement of the perfusion/aortic pressure. 
Please refer to the schematic diagram below.
NOTE: the second transducer (with a balloon catheter) on the right hand side of the diagram is inserted into the left ventricle of the heart to measure left ventricular pressure.
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