How do I measure the coronary artery flow within a working heart preparation?

Coronary flow in a Working Heart preparation can be determined on the following basis:
 

aortic flow + coronary flow = total flow
 

If the perfusate is NON-RECIRCULATING, the coronary flow can be determined as follows:

  • The Atrial Flow is already known by setting the peristaltic pump.
  • The Aortic Flow is collected for a precise time period (as the perfusate exits system via waste line) and the volume is carefully measured.
  • Likewise, the perfusate that drips from the heart is supplied by the coronary arteries.
    • This volume can be collected over a precise time period and the volume carefully determined.
      This will provide a measure of Coronary Flow.
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