Coronary flow in a Working Heart preparation can be determined on the following basis:
aortic flow + coronary flow = total flow
If the perfusate is NON-RECIRCULATING, the coronary flow can be determined as follows:
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The Atrial Flow is already known by setting the peristaltic pump.
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The Aortic Flow is collected for a precise time period (as the perfusate exits system via waste line) and the volume is carefully measured.
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Likewise, the perfusate that drips from the heart is supplied by the coronary arteries.
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This volume can be collected over a precise time period and the volume carefully determined.
This will provide a measure of Coronary Flow.
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