In this webinar, Dr. Melanie White presents the various methods for assessing cardiac function in the context of pathology, spanning from in vitro to in vivo techniques, and how she integrates these with cutting-edge mass spectrometry in her research on cardiovascular disease pathogenesis.
Understanding the dynamics of the heart and how it changes with pathology is a question that can be addressed from many angles. No matter which way you look at it, the best approaches must start by selecting an appropriate method to assess cardiac function that permits both real-time assessment and downstream analysis. From in vitro preparations, including isolated cardiomyocytes, stem cells and cardiac organoids; to ex vivo approaches using Langendorff and working heart systems, and finally; in vivo, whole system approaches, the approaches are as varied as the outcomes. Dr Melanie White will discuss not only these different methods to assess the pathogenesis of cardiovascular disease but how she approaches this aspect of her research to align with cutting edge mass spectrometry.
Key Topics Include:
- Method considerations to address different biological questions
- Top tips and tricks for improving success using ex vivo cardiac perfusion (from 20+ years of experience)
- What are the downstream implications for the various approaches to basic cardiac functional studies
Presenter
Melanie White, PhD, FAHA
Heart Foundation Future Leader Fellow, School of Medicine, The University of Sydney
Dr. Melanie White (FAHA) is the PI of the cardiometabolic proteomics group in the Charles Perkins Society at the University of Sydney. With over 20 years of experience in pre-clinical models of cardiovascular disease and cutting-edge mass spectrometry, her group research goals include understanding more about how cells adapt to their changing environment by altering proteins using post-translational modifications.