There are two options for connecting the Millar Electrophysiology catheter (EPR-800, EPR-801, or EPR-802) to the FE238 Octal Bio Amp as detailed below:
Monopolar Recordings
For monophasic cardiac action potentials, you will need two linking cables, the MLAC49 and MLAC29. First, connect the linking cables to the ground and negative inputs of the Octal Bio Amp, marked by the blue circles in the image below. The connections to the discontinued ML138 Octal Bio Amp are identical. The double-sided connector of the MLAC49 will not be connected at this time. Connect up to eight 1.5 mm touchproof connectors on the EPC-5a Cable to the appropriate positive inputs on the Octal Bio Amp, colour coded in the image below.
Bipolar Recordings
To record biphasic action potentials, connect the desired electrode pairs to the positive and negative inputs on the Octal Bio Amp's front panel (one pair per channel). This method allows for the use of up to four amplifier channels.
If cardiac pacing is required, select two electrodes for stimulation. Connect the corresponding EPC-5a cable connectors to the provided white pin adapters. Then, use the stimulator clip electrodes to link these to the Isolated Stimulator.
Attach an SP0134 Earth Cable to the grounding stud on the PowerLab's rear. Connect the alligator clip at the cable's other end to the bare green wire in the EPC-5a Cable's wiring harness. This green wire is linked to the EPC-5a's wiring harness shield, and grounding it will help reduce electrical noise in the signal.
For in vivo applications using an EPR catheter with disposable, snap-connect ECG electrodes, connect an MLA0313 Lead Wire to either the MLAC49's double-sided connector or directly to the amplifier's common input. Attach the lead wire to an electrode on the animal's prepared ventral (belly) side.
For ex vivo applications, connect an MLA1505 Lead Wire to the MLAC49's double-sided connector. Clamp this to a stripped silver wire wrapped around a cannula and its associated vessel. Once implanted, the EPR catheter can be connected to the EPC-5a Interface Cable for data recording.
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