The preferred protocol for recording earthworm action potentials uses the Bio Amplifier built into T-Series PowerLabs
In our experience, the earthworm action potential protocol requires the additional amplification provided by this amplifier. Signal falls off at the square of the distance from the source; thus, additional amplification when using the intact prep is recommended.
Some customers have attempted to record the action potential using pod port input cables (MLA255 or MLA285
) attached to needles in the worm with mixed results. This approach works best if you fully dissect the nerve cord from the worm and use the MLT016 Nerve Chamber
, placing the nerve in the chamber directly touching the stainless steel wires. Note: amplitudes of the action potentials in the recorded from earthworm will be significantly smaller than the compound action potentials from sciatic nerve from a frog or mammal.