Can I calibrate an ML206 Gas Analyser with a mixture of 0% O2 and 0% CO2 as one of the known gases?

The gases sampled into the ML206 Gas Analyser requires a minimum concentration of 5% O2 in order for the O2 sensor to function. Therefore using a mixture that contains less than this concentration (such as pure N2) will not result in an accurate calibration or accurate readings.