What quantity of blood is required when calibrating Volume using the Rho Cuvette?

When using the MPVS Ultra for large animal models, you will need to calibrate using Baan's equation which requires a predetermined alpha value and a measurement of the blood resistivity using the Rho Cuvette.
Approximately 0.5ml of blood will be required to fill the well in the Rho Cuvette (hence this is not a suitable calibration method for smaller animals).