Using the DMT wire myography system for microvessel research is a gold standard, but like any fine research instrument it requires diligent preparation ahead of time. Calibration of the instrument prior to acquisition should be part of any user's experimental protocol. ADInstruments provides an easy method for the calculation of optimal pretension conditions for microvascular or any small tubular tissue research using the DMT Normalization Module. The rationale and theory behind normalizing elastic tissues such as microvessels has been extensively investigated by Professor Michael Mulvany (for details see Mulvany MJ, Halpern, W Circulation Research 1977, 41: 19-26). Mulvany determined that the optimal pre-tension conditions (i.e. internal circumference) for microvascular studies is best defined as the size when the vessel is fully relaxed and under a transmural pressure of 100 mmHg. Therefore the aim of the normalization procedure is to determine the internal circumference at which the vessel would be relaxed and under a transmural pressure of 100 mmHg. This index is denoted as IC100 and is calculated for each vessel mounted on the wire myograph. The DMT Normalization Module allows the user to enter different micrometer settings that involve sequential stretching of the tissue and recording of the resultant force exerted by the vessel walls using LabChart.
As of July 14 2017, ADInstruments no longer sells or supports any DMT products (unless previously purchased under tender contract). Instead please contact DMT direct.