Radnoti Oxygenating Chamber (Sheet Flow) 

An oxygenating chamber that is designed to be vertically attached in a Radnoti Working Heart apparatus for rat hearts. Its upper chamber is a condenser, which is formed from six linked spheres that provide the maximal surface area for rewarming and reoxygenation of the perfusate. Perfusate passing through the condenser is collected in its lower chamber, which is designed to collect the perfusate. It has a port that allows the insertion of an Oxygenating Tube with Teflon Needle Valve to provide controlled gas delivery. Additionally, it has another specialized port for the option of inserting either a temperature or pH probe to monitor temperature or pH.

This oxygenating chamber has seven Luer ports, one entry port at the top, three side ports near the top of the upper chamber, two side ports connecting to the lower chamber, and one exit port at the bottom. Attachment of either 1- or 3-Way Luer Taps allows the top and side ports to be either used for entry or exit of perfusate overflow, perfusate return (for working heart mode), gassing, perfusate sampling or addition of drugs. The bottom exit port permits the reoxygenated perfusate to be pumped back to the isolated heart. It has a 160 mL volume, 370 mm overall average height and 48 mm water jacket OD.

Radnoti Oxygenating Chamber (Membrane) 

An oxygenating chamber that is designed to be horizontally attached to a Radnoti Lab Stand via two Single Ring Clamps, which is then used to gas solutions that are being perfused at very low flow rates required for certain organs, tissues, and cells. They include Isolated Rodent Liver/Kidney and Mouse Langendorff and Working Heart setups.

The oxygenating chamber has a lid that seals an inner incubating chamber, and has gas inflow and outflow ports, which allow the chamber to be gassed from a gas supply (depending on the user and laboratory facilities). The gas pressure should not be too high, but within the recommended range, which is similar to a gentle stream when played over the hand. Attached to the lid is a three rod cylindrical frame that is wrapped by a coil of silicone tubing, which is highly permeable to oxygen, carbon dioxide and most other common gasses. Therefore, firstly the chamber should be allowed to come to temperature, before passing the perfusate through the silicone tubing, which permits rapid gas equilibration with most gas mixtures.