How do I calibrate a Micro Oxygen Electrode?

Electrode Assembly and Preparation


  1. Remove the acrylic housing from the Oxygen electrode by unscrewing it from the electrode body. This housing, with affixed Teflon membrane, does not contain the necessary oxygen electrolyte solution.
  2. Add oxygen electrolyte solution to the acrylic housing. This is accomplished by attaching a filling tip to the bottle of electrolyte and adding the electrolyte to the housing to a minimum height of 6 mm. Gently place the bubble-free end of the filling tip against the Teflon membrane and release the electrolyte to the minimum height.
  3. Insert the housing on to the Oxygen electrode, being careful not to trap any air bubbles near the electrode tip. The housing is screwed clockwise into the body of the electrode until it stops. Check the tip of the electrode for proper seating of the housing against the electrode. The electrode is properly seated if the electrode protrudes slightly beyond the end of the housing.


Calibration of the electrode requires the use of two standard gases with percent values that are close to the percent values of Oxygen to be measured. Common values used are 0% Oxygen for zeroing and 21% Oxygen (Ambient Air) for sloping or gain.


A. Calibration for gas samples:

When samples to be measured are gaseous, then calibration should be performed with humidified gases. Two possible setups for calibrating the electrode are shown below. Keep the tip of the electrode as far as possible from the surface of the water. The bubbling rate of the gas should be slow (3 - 6 bubbles per second). Although bubbling at a faster rate will flush the chamber more quickly, it will also cause a cooling effect on the electrode.


  1. Bubble the 0% gas through the chamber and adjust the zero of the meter after a stable reading is obtained. It may take up to 15 minutes to de-gas the chamber of contaminants, however bubbling the gas vigorously will cause droplets to collect on the tip of the electrode. This will make the electrode response time appear to be slow.
  2. Bubble the 21% gas (or any other percent value you decided to use for your application) through the chamber until a stable reading is obtained. Adjust the calibration control to 21%.
  3. This procedure of alternating between the two gases should be continued until you become confident of stability and reproducibility. The electrode is now ready to use.

B. Calibration for liquid samples

To decrease calibration time, two separate calibration chambers should be used. One for the 0% gas and another for the sloping gas such as 21%. When setting up the calibration chambers initially, it will take up to 30 minutes to flush each chamber to obtain a steady state oxygen level and a constant temperature. Again the bubbling rate should be carefully regulated (3 - 6 bubbles per second) so that both calibrating liquids are at the same temperature.

Calibrating standards and samples must be at the same temperature for accurate Oxygen measurements.



  1. Immerse the tip of the electrode into the 0% standard and adjust the zero of the meter after a stable reading is obtained.
  2. Remove the electrode from the first standard and place it into the second standard. Adjust the calibration control to the value of the second standard (ex: 21%).


Alternate between the two standards until you become confident of stability and reproducibility. The electrode is now ready to use.


NB If the elctrode is slow to respond, it may be that the membrane not screwed on tight enough. It should be tightened to the point the glass tip touches the membrane material and pushes it less than 1 mm.


For further technical assistance with this or any other issue, please contact ADInstruments Technical Support by clicking HERE.