The rodent Langendorff was redesigned with user needs in mind
In this excerpt from her latest Langendorff webinar, Dr Melanie White, an isolated heart expert from the University of Sydney, explores her favorite features of the Rodent Langendorff and Direct Perfusion Core.
Related: Why we redesigned the Langendorff Apparatus
Melanie’s Favorite Features of the Direct Perfusion Core
- Four sensor ports
- Ensures fluid measurements reflect the point immediately before the cannula
- Effective recirculation
- Prevents buffer degassing
- Perfusate is never stagnant in the lines
- Maintains the narrow ranges of pH and oxygen saturation required
- Reduces buffer wastage
- Reduced dead volume
- The perfusate has less distance to travel to get to the heart
- Saves around 2 mins in the perfusion protocol
- Built-in bubble trap
- Eliminates bubbles in the perfusate without fuss
- Effective heat exchange
- The buffer is being transported to the heart in the most optimal way
Related: Dr Melanie White - Modernising the Langendorff
Melanie’s Favorite Features of the ADInstruments Rodent Langendorff
- Compact design
- A modular design that fits easily on a benchtop and under shelves
- Built-in bubble trap
- Eliminates bubbles without fuss
- Borosilicate glass
- Durable, autoclavable, and provides a clear line of sight long-term.
- Closed reservoirs
- Minimises dust contamination
- Spill tray
- Keeps the system, and the lab, clean
- More data collected
- It’s easier to spot potential outliers
- Buffer recirculation
- Reduces waste, and is cost effective
- Reduced dead volume between heart and reservoirs
- Less distance for the perfusate to travel, keeping the heart happy!
Related: Dr Melanie White - The History and Principles of Langendorff
Dr. Melanie White
ARC DECRA Research Fellow
School of Medicine
University of Sydney
About the speaker...
Dr. Melanie White is a member of the Charles Perkins Society, a Heart Foundation Future Leader Fellow and teaches senior students in the discipline of Pathology at the University of Sydney School of Medicine.
Her research interests include understanding more about how cells adapt to their changing environment by altering proteins using post-translational modifications. Her team’s work centers on asking these questions in clinically relevant models of myocardial ischemia (heart attack), type 2 diabetes and obesity.