Radnoti Water Jacketed Flex Tubing Assemblies

Ensuring that the perfusate is maintained at the desired temperature can be extremely important, especially in isolated organ studies. However, while the glass components are water jacketed against heat loss, it is common that the connecting tubing is not water jacketed. Hence, there will be heat loss that occurs during perfusate transfer through tubing, especially during slow flow rates, which can be several degrees centigrade, before it reaches the study sample. It can be further compounded in some situations, such as perfusate flow being affected by coronary regulation in an isolated heart preparation.

Therefore, the Radnoti Water Jacket Tubing Assembly is designed to address the issue by providing complete water jacketing to the transfer tubing. Essentially it is a tubing within a tubing configuration, where the outer Tygon tubing (0.375" ID x 0.5" OD) connects to the heated water perfusion circuit via Radnoti Quick Disconnect fittings. An inner transfer tubing (2 mm ID x 3 mm OD) connects to a Radnoti QD to Flex Tube Ferrule Adapter with an O-Ring, which is then secured onto a glass body via a Drilled Cap. To ensure a good seal, the threaded part of the adapter is wrapped around at least twice with 12mm x 10m x 0.1mm standard thread seal (PTFE) tape (not supplied) and its end attaching to a Radnoti Flex Tube Ferrule. A Radnoti Flex Tube Thread to Male Luer Adapter screws on top and its other end attaches to a Finger Snap Luer Lock Ring.

The Radnoti Water Jacket Tubing Assembly is available in a range of lengths, which are used in a range of perfusion setups, including Isolated Rodent Liver/Kidney, Langendorff and Working Heart. Replacement components are also available in packs.

Image