Radnoti Water Jacketed Flex Tubing Assemblies
Ensuring that the perfusate is maintained at the desired temperature can be extremely important, especially in isolated organ studies. However, while the glass components are water jacketed against heat loss, it is common that the connecting tubing is not water jacketed. Hence, there will be heat loss that occurs during perfusate transfer through tubing, especially during slow flow rates, which can be several degrees centigrade, before it reaches the study sample. It can be further compounded in some situations, such as perfusate flow being affected by coronary regulation in an isolated heart preparation.
Therefore, the Radnoti Water Jacket Tubing Assembly is designed to address the issue by providing complete water jacketing to the transfer tubing. Essentially it is a tubing within a tubing configuration, where the outer Tygon tubing (0.375" ID x 0.5" OD) connects to the heated water perfusion circuit via Radnoti Quick Disconnect fittings. The inner transfer tubing (2 mm ID x 3 mm OD) connects to an O-ring adapter that is inserted through a polypropylene cap, and attaches to a male Luer with a lock ring connector. The cap screws onto a glass body, which tapers down for the outer Tygon tubing to fit over it.
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