ADInstruments Rodent Cardiomyocyte Isolation Apparatus
Overview
The ADInstruments Rodent Cardiomyocyte Isolation Apparatus is a modular and compact unit that is designed to function with a suitable peristaltic pump and heated water circulator, such as the Minipuls 3 Peristaltic Pump and Thermal Water Circulator (5L). Together they are ideal for extracting cardiomyocytes from isolated rodent hearts (mice and rats) via retrograde perfusion of an enzymatic solution. The apparatus incorporates high-quality stainless steel and double-walled, borosilicate glassware that provides clear visualization of the entire perfusate pathway for detecting air bubbles or any other contaminants. The standard design can allow up to two consecutive perfusions of different enzymatic solutions. One of the enzymatic solutions can be further recirculated with the Minipuls 3 Peristaltic Pump.
The apparatus can be easily simplified or upgraded to match the complexity of your experimental needs. Please consult your local sales representative for more information.
Components
- Steel Base, two Stainless Steel Support Rod (24”), a Stabilizer Support Bar (5.5”), and an Aluminium Rod (8”)
- One 1.0 L Water Jacketed Reservoir and Oxygenating Bubbler with Inlet
- Reservoir Filling Funnel
- Two Enzyme Buffer Reservoirs
- Heating Coil w/ Bubble Trap (10 mL)
- Cardiomyocyte Isolation Heart Chamber
- Four different sizes of Aortic Cannula Tips (22, 20, 16 and 14 Gauge)
- Ring Clamps, six Universal Stand Clamps (Stainless Steel) and two Universal Stand Clamps (Nylon)
- Stand Base Spill Tray (3 Rod)
- 20 mL Damping Syringe
- Tubing Adapter Kit
- Tubing and accessories
Accessories (purchased separately)
Related
Products
Research Applications
Cardiovascular Isolated Heart Systems
In Vitro Pharmacology + Isolated Tissue and Organs
Signals
Key Features
- Modular construction enables easy personalization of various glass components and accessories
- RoHS-compliant corrosion-resistant stainless steel base and supports
- High-quality borosilicate glassware that is easy to clean and autoclavable, while providing clear and easy inspection of the perfusion circuit at all times
- Seamless switching between Krebs–Henseleit buffer and enzyme buffer reservoirs
- Tested water-jacketed perfusion circuit that prevents heat loss and maintains uniform temperature
- A heating coil with bubble trap compliance chamber for trapping any air bubbles and with a damping syringe reduces pressure oscillations due to a pump
- An overflow outlet that allows for buffer re-oxygenation during any waiting time
- A locking male luer outlet that provides a direct and secure attachment to an aortic cannula tip, while maintaining easy interchangeability
- Compatible with four different sizes of Aortic Cannula Tips
- Can attach to own customized aortic luer cannula
- Provides the option to recirculate enzymatic solution with suitable peristaltic pump
- Fine controlled and uniform buffer gassing in the buffer and enzyme buffer reservoirs
- High-quality Tygon tubing that prevents degassing of the perfusate
- A sturdy spill tray that drains away spills and other fluid messes
Support
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