Langendorff
Langendorff Isolated Heart Perfusion
With the Langendorff isolated heart perfusion technique you can monitor an isolated heart while perfusing the coronary arteries with a nutrient solution. This allows you to record and analyze multiple cardiac parameters such as left ventricular developed pressure, perfusion pressure, cardiac electrical activity, heart rate and temperature all in real time.
ADInstruments' solutions support your use of the Langendorff animal heart perfusion technique to perfuse all sizes of heart models, from mice, rats, and guinea pigs, to porcine hearts.
Products and Solutions
Record multiple
signals at one time
Compact and modular options available
Mix and match products
for a custom solution
Integrate all your data
streams in one place
- Perfuse all sizes of heart models hearts from mice, rats and guinea pigs, to porcine hearts
- Choose from a compact system which utilises a peristaltic pump and STH pump controller for constant flow and pressure or more traditional modular system
- Maintain constant temperature with a heater water bath, water jacketed tubing, components and heart chamber
- Easily switch between constant-pressure and constant flow modes to investigate coronary artery function while the heart beats spontaneously or with the help of an external stimulator
In conjunction with our complete Langendorff perfusion systems shown below, any device with an analog output (+/- 10V) can be connected to a PowerLab data acquisition system for synchronization of event in LabChart. So you have even more flexibility and can integrate all your data streams in one place.
All of our hardware is backed by the appropriate safety certification standards. More information on safety standards.
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Isolated Heart Action Potential KitsIsolated heart kits that allow surface cardiac action potential ("ECG") measurements from an isolated perfused rodent heart in Langendorff and Working Heart systems.
PowerLab C Rodent Langendorff Foundation SystemThe PowerLab C Rodent Langendorff Foundation System is designed to perfuse an isolated mouse or rat heart, either in constant perfusion pressure or constant perfusion flow. Included is the high-quality, modular, yet compact ADInstruments Rodent Langendorff Apparatus, which provides flexibility for configuring various isolated heart experimental solutions. Additionally, the system is also supplied with LabChart Lightning software, flexible PowerLab C data acquisition, and hardware for measuring perfusion pressure and temperature. Further tailored parameters can be made by additionally purchasing from a range of compatible isolated heart kits, which includes measurements of left ventricular developed pressure, heart rate and biopotentials.
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Perivascular and Tubing Flow SystemsOne or Two Channel Perivascular/Tubing Flow Systems can be used to measure perivascular blood flow in a single blood vessel from a range of animal species as well as measuring tubing fluid flow.
Langendorff Systems - Radnoti (Constant Pressure or Flow)Langendorff Foundation Systems that provide high quality glassware to perfuse an isolated mouse or rat heart in Langendorff mode with a versatile PowerLab and LabChart data acquisition and analysis system. Langendorff Isolated Heart Systems can be tailored to measure a number of parameters including pressure, biopotentials and temperature via a wide range of kits, available separately.
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Isolated Heart Pressure KitsIsolated Heart Pressure Kits are hardware add-ons for Langendorff and Working Heart Foundation Systems. Each kit allows the measurement of pressure for a specific application.
Isolated Heart Pacing KitsThe Isolated Heart Pacing Kit is an add-on that allows surface pacing/stimulating of an isolated rodent heart in the Langendorff and Working Heart foundation systems.
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Isolated Perfusion Temperature & pH KitA perfusion kit that is designed to measure pH and temperature in any appropriate and meaningful location of an isolated perfusion circuit such as the Langendorff and Working Heart systems.
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LabChart LightningData acquisition and analysis re-imagined. Make unique discoveries with unlimited freedom and flexibility.
StimulatorsSoftware-controlled, constant-current stimulators designed to work with a C Series Front End Interface or traditional PowerLab (purchased separately) for direct or field stimulation of in vitro tissue samples. Additionally, the isolated stimulator option can also be used for in vivo animal and human studies involving nerve and muscle stimulation.
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Radnoti Langendorff Constant Perfusion Flow ApparatusesDesigned to maintain cardiac function of isolated heart preparations in constant perfusion flow Langendorff mode, and are available in recirculating and non-recirculating configurations. Several sizes are also available to accommodate different heart sizes including mouse, rat, guinea pig, rabbit or cat.
Radnoti Langendorff Constant Perfusion Pressure ApparatusesDesigned to maintain cardiac function of isolated heart preparations in gravity-fed constant perfusion pressure Langendorff mode, and are available in recirculating and non-recirculating configurations. Several sizes are also available to accommodate different heart sizes including mouse, rat, guinea pig, rabbit or cat.
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Radnoti Thermal Bath/CirculatorThis digital, heated circulator allows temperature control from ambient temperature to 120°C. Ideal for the bench top.
Minipuls 3 Peristaltic PumpA reliable, pulse-free peristaltic pump providing ultra-smooth flow.
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ADInstruments Rodent Langendorff ApparatusThe ADInstruments Rodent Langendorff Apparatus is relatively compact yet modular, which provides flexibility when configuring various isolated heart experimental solutions. This high-quality stainless steel and borosilicate glass apparatus includes an ADInstruments Direct Perfusion Core, which allows recirculation and reoxygenation of perfusate after priming of the apparatus, and measurements to be made close to the cannulated heart.
Physiological Pressure TransducerThe Physiological Pressure Transducer is a highly accurate and robust piezo-resistive transducer.
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Non Invasive Pulse TonometerThe Non Invasive Pulse Tonometer is a hand-held wand probe equipped with a Mikro-Tip pressure sensor at the tip, designed for non-invasive recording of pulse pressure wave contours.
Transonic Flowprobes and FlowsensorsA range of Flowprobes and Flowsensors are available to measure fluid flow in laboratory tubing; blood flow in vessels of various diameters; and cardiac output in acute and chronic preparations.
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T-type Thermocouple ProbesT-type Implantable Thermocouple Probes are suitable for connection to the T-type Pod or Cardiac Output Pod.
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Tuohy Borst Adapters (pack of 10)A pack of replacement Tuohy Borst Adapters for use with the Cardiac Output, Langendorff or Working Heart Systems.
Peristaltic TubingTwelve lengths of SMA flow-rated tubing for use with the Peristaltic Pump.<
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STH Pump Controller to Peristaltic Pump Cable IIConnects the STH Pump Controller to the input Minipuls 3 Peristaltic Pump to maintain constant pressure in a compatible Langendorff apparatus.
Spring Clip ElectrodesThe Spring Clip Electrodes are ideal for measuring electrical activity from the isolated heart in a Langendorff or Working Heart preparation.
Flexible Data Acquisition
ADInstruments systems provide an integrated solution to advance life science research. With the combination of LabChart or LabChart Lightning analysis software and a PowerLab data acquisition unit, you have the flexibility to collect and synchronize a wide range of signals for analysis. We also offer a range of LabChart compatible solutions able to stream directly in LabChart.
LabChart data analysis software creates a platform for all of your recording devices to work together, allowing you to acquire biological signals from multiple sources simultaneously and apply advanced calculations and plots as your experiment unfolds.
Data acquisition and analysis re-imagined. Make unique discoveries with unlimited freedom and flexibility.
High-performance data acquisition hardware designed for life science research. PowerLab is engineered for precise, consistent, reliable data acquisition, giving you the reproducible data you need while meeting the strictest international safety standards.
A modular data acquisition foundation system that provides unparalleled flexibility for researchers looking to invest in customizable, reliable solutions for both now, and in the future.
All your analysis in one place
LabChart software is designed specifically for life science data and provides up to 32 channels for data display and analysis options that are powerful and easy to use. With auto-recognition of ADI and LabChart Compatible hardware, multi-window views, one touch recording, simultaneous recording from multiple devices, specialized preconfigured settings, easy sharing options and an interface that can be customized to show only the features you want to use.
Features and Add-Ons
Additional acquisition and analysis options to support your Langendorff analysis:
Data acquisition and analysis re-imagined.
Make unique discoveries with unlimited freedom and flexibility.
