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Langendorff

Langendorff

Langendorff Isolated Heart Perfusion

With the Langendorff isolated heart perfusion technique you can monitor an isolated heart while perfusing the coronary arteries with a nutrient solution. This allows you to record and  analyze multiple cardiac parameters such as left ventricular developed  pressure, perfusion pressure, cardiac electrical activity, heart rate and temperature all in real time.

ADInstruments' solutions support your use of the Langendorff animal heart perfusion technique to perfuse all sizes of heart models, from mice, rats, and guinea pigs, to porcine hearts.

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Langendorff

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Products and Solutions

Record multiple<br /> signals at one time

Record multiple
signals at one time

Compact and modular options available

Compact and modular options available

Mix and match products<br /> for a custom solution

Mix and match products
for a custom solution

Integrate all your data<br /> streams in one place

Integrate all your data
streams in one place

  • Perfuse all sizes of heart models hearts from mice, rats and guinea pigs, to porcine hearts
  • Choose from a compact system which utilises a peristaltic pump and STH pump controller for constant flow and pressure or more traditional modular system
  • Maintain constant temperature with a heater water bath, water jacketed tubing, components and heart chamber
  • Easily switch between constant-pressure and constant flow modes to investigate coronary artery function while the heart beats spontaneously or with the help of an external stimulator

In conjunction with our complete Langendorff perfusion systems shown below, any device with an analog output (+/- 10V) can be connected to a PowerLab data acquisition system for synchronization of event in LabChart. So you have even more flexibility and can integrate all your data streams in one place.

All of our hardware is backed by the appropriate safety certification standards. More information on safety standards.

Complete System

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Components & Related Products

Typical Langendorff Studies

Isolated heart and organ perfusion

Medical device testing

Artificial heart development

Ischemia / Reperfusion studies

Pharmacology / toxicology studies

Myocardial function

Hypertrophy, infarction, cardiomyopathy and other disease models

Systemic circulation or ventricular studies

Coronary vessel function

Signals Supported

Dissolved Gases
+

Easily detect gas thresholds with graphical aids like LabChart's Guide Lines and the DVM mini window, and use the Event Manager Add-On to automate the detection of gas thresholds.

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dP/dT
+

dP/dt is a signal derived from pressure, typically ventricular blood pressure, that indicates the change in pressure over time.

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ECG
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Cardiac electrical activity from the myocardium can be monitored to assess myocardial damage to electrophysiological workings of the heart.

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Heart Rate
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Using pressure or electrical activity, monitor heart health by calculating heart rate.

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Pressure
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Coronary perfusion pressure and left ventricular developed pressure can be monitored during an isolated heart session to gauge myocardial performance.

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Temperature
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Ensure the maintenance of a proper physiological environment in your system, or investigate the impacts of temperature on performance.

More info »

Flexible Data Acquisition

ADInstruments systems provide an integrated solution to advance life science research. With the combination of LabChart or LabChart Lightning analysis software and a PowerLab data acquisition unit, you have the flexibility to collect and synchronize a wide range of signals for analysis. We also offer a range of LabChart compatible solutions able to stream directly in LabChart.

LabChart

LabChart

Enabling Discovery

LabChart data analysis software creates a platform for all of your recording devices to work together, allowing you to acquire biological signals from multiple sources simultaneously and apply advanced calculations and plots as your experiment unfolds. Learn more about LabChart and Langendorff »

LabChart Lightning LabChart Lightning

Data acquisition and analysis re-imagined. Make unique discoveries with unlimited freedom and flexibility. Try LabChart Lightning »

PowerLab

PowerLab

Data with Integrity

High-performance data acquisition hardware designed for life science research. PowerLab is engineered for precise, consistent, reliable data acquisition, giving you the reproducible data you need while meeting the strictest international safety standards. Learn more »

PowerLab C

PowerLab C

Re-engineered for
unparalleled flexibility

A modular data acquisition foundation system that provides unparalleled flexibility for researchers looking to invest in customizable, reliable solutions for both now, and in the future. Learn more »

All your analysis in one place

LabChart software is designed specifically for life science data and provides up to 32 channels for data display and analysis options that are powerful and easy to use. With auto-recognition of ADI and LabChart Compatible hardware, multi-window views, one touch recording, simultaneous recording from multiple devices, specialized preconfigured settings, easy sharing options and an interface that can be customized to show only the features you want to use.

  • Typical 'lang' recording highlighting LVP, ECG, perfusion pressure and calculated variables. The Blood Pressure Add-On analyzes ventricular pressure waveforms for you.

    Typical 'lang' recording highlighting LVP, ECG, perfusion pressure and calculated variables. The Blood Pressure Add-On analyzes ventricular pressure waveforms for you.
Pro Tip
Pro Tip: Measuring aortic pressure in a Langendorff Heart preparation

Measuring aortic pressure in a Langendorff Heart preparation

'Perfusion pressure' and 'aortic pressure' are two terms used to describe one of the pressure parameters in a Langendorff Heart experiment.

To measure aortic pressure in a Langendorff heart preparation the perfusate in the perfusate reservoir is typically gassed with 95% O2 and 5% CO2 for at least 30 minutes. The perfusate then flows into the aorta of the heart via a 3-way tap. The catheter of the pressure transducer is also connected to the 3-way tap, which allows constant measurement of the perfusion/aortic pressure. 

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Features and Add-Ons

Additional acquisition and analysis features and add-ons to support your Langendorff studies:

Blood Pressure LabChart Pro
+

Automatically detects, analyzes, and reports cardiovascular parameters from arterial or ventricular pressure signals in real time or post acquisition. 

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Blood Pressure

MLS370/8

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ECG Analysis LabChart Pro
+

The ECG Analysis Module for LabChart automatically detects and reports PQRST onset, amplitude and intervals in real time, or after recording.

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ECG Analysis

MLS360/8

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Dose Response LabChart Pro
+

Simultaneously monitor multiple cardio-mechanic and cardio-energetic parameters as well as ECG signal then automate valuable calculations in the dose response module including EC50 values, Hill slopes, and additional parameters. 

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Dose Response

MLS390/8

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HRV LabChart Pro
+

The Heart Rate Variability (HRV) Module for LabChart analyzes beat-to-beat interval variation in ECG recordings or arterial pulse signals.  

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HRV

MLS310/8

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Peak Analysis LabChart Pro
+

Automate calculation of peak parameters such as peak height, half-width, spike height, rise time, area and tau.

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Peak Analysis

MLS380/8

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Macros
+

Can be used to automate commands during recording. Record your actions for repetitive operations, or get under the hood and write custom processes in VB Script.

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Macros

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Stimulator Dialog
+

Create a custom protocol to control stimulation frequency and timing of pulse trains.

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Stimulator Dialog

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LabChart Lightning
LabChart Lightning
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Data acquisition and analysis re-imagined.

Make unique discoveries with unlimited freedom and flexibility.

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Videos

57:00

Experimental Considerations and Best Practices when using the Isolated Langendorff Heart Technique

Learn about core principles and applications of the Langendorff perfusion technique as well...

41:05

Using the ECG Analysis, HRV and Cardiac Axis tools

For anyone interested in adding to their stock of cardiovascular data analysis knowledge, this...

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Training

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Surgical Training

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Training »

Related Support Articles

Experimental Considerations and Best Practices when using the Isolated Langendorff Heart Technique
Panlab Langendorff - Langendorff features
Langendorff System Overview - Panlab (Constant Pressure or Flow)
How do I maintain the Gas diffusers in my PanLab Langendorff/Organ Bath
What are the cleaning guidelines for the Panlab Langendorff System?
more

Related Application Areas

Invasive Blood Pressure (animal)

Ventricular Pressure Volume

Invasive Blood Flow

NIBP (animal)

Working Heart

Pharmacology

See all Cardiovascular applications »

Citations

2,400 papers

in Langendorff on Google Scholar cite ADInstruments.

Fiona J Sutherland, Michael J Shattock, Kathryn E Baker, David J Hearse, Mouse isolated perfused heart: characteristics and cautions

Related Blog Articles

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Testimonial

ADI has an excellent group that does much more than deliver research material. There is excellent service to advise what is the most proper equipment before sale; installation, adjustments and training at the time of the beginning of activities and the support for adaptations in the after-sale. This activity was an essential differential for the kind of work I do. Currently, ADI presents itself as a research partner of its customers, rather than a suppliers of products.

Dr. Cléo Leite

Federal University of São Carlos. Brazil

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Thank you for your interest in Langendorff. Please complete and submit the form below and an ADInstruments representative will aim to get in touch within one working day. If you’d like to speak to someone immediately, office contact information is available from the contact page.

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